Three-dimensional culture method induces the chondrogenic differentiation of human adipose-derived mesenchymal stem cell microspheres

doi:10.3969/j.issn.2095-4344.2015.01.005

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (1): 24-29.doi: 10.3969/j.issn.2095-4344.2015.01.005

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Three-dimensional culture method induces the chondrogenic differentiation of human adipose-derived mesenchymal stem cell microspheres

Bi Xiao-juan¹, Guo Chen-ming², Li Liang¹, Duan Xian-lin³, Jiang Ming^{1, 3}

¹Stem Cell Laboratory, Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China;²Department of Breast Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; ³Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Research Institute of Hematology, Urumqi 830054, Xinjiang Uygur Autonomous Region, China

Revised:2014-12-08 Online:2015-01-01 Published:2015-01-01
Contact: Jiang Ming, Master, Chief physician, Professor, Doctoral supervisor, Stem Cell Laboratory, Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Research Institute of Hematology, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
About author:Bi Xiao-juan, Assistant researcher, Stem Cell Laboratory, Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
Supported by:
the Special Fund for Tissue Engineering in the First Affiliated Hospital of Xinjiang Medical University, No. 2012ZZGC04

Abstract

Abstract:

BACKGROUND: Adipose-derived mesenchymal stem cells are more appropriate as seed cells for cartilage induction in patients who have unsatisfied repair after articular cartilage injuries, but how to induce the cartilage function need to be studied.
OBJECTIVE: To induce the chondrogenic differentiation of human adipose-derived mesenchymal stem cell microspheres using three-dimensional culture system.
METHODS: Adipose tissues were extracted after liposuction under sterile, to isolate and culture human adipose-derived mesenchymal stem cells. The third passage cells were analyzed by flow cytometry and identified by osteogenic induction and adipogenic induction, and meanwhile, cells were cultured under three-dimensional culture system for chondrogenic differentiation. Then, cells were observed by Alcian blue staining for identification of glycosaminoglycan synthesis as well as hematoxylin-eosin staining for histological analysis. Expression of collagen type II was detected by immunofluorescence method. Cartilage stiffness was determined by mass measurement.
RESULTS AND CONCLUSION: Isolated human adipose-derived mesenchymal stem cells highly expressed CD105, CD44, CD29, but showed low expressions of CD45, CD34. After osteogenic and adipogenic induction, cells were positive for alizarin red staining and oil red O staining. Under the three-dimensional culture, chondrocytes could greatly express glycosaminoglycans and collagen type II. The results suggest that under the three-dimensional culture, human adipose-derived mesenchymal cells differentiate into chondrocytes and have the characteristics of chondrocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Tissue Engineering, Stem Cells, Mesenchymal Stem Cells, Cartilage

CLC Number:

R394.2

Bi Xiao-juan, Guo Chen-ming, Li Liang, Duan Xian-lin, Jiang Ming. Three-dimensional culture method induces the chondrogenic differentiation of human adipose-derived mesenchymal stem cell microspheres[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 24-29.

Figures/Tables 4

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Three-dimensional culture method induces the chondrogenic differentiation of human adipose-derived mesenchymal stem cell microspheres

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