Primary culture and identification of kitten extraocular muscle myoblasts

doi:10.3969/j.issn.2095-4344.2014.51.002

Abstract

Abstract:

BACKGROUND: Strabismus models need to be made in senior mammals with certain binocular visual function and eyes located forward, such as Felidae. Myoblasts of cat extraocular muscle via in vitro isolation and cultivation are with characteristics including high purity, strong vitality, and uniform biological characteristics, which are critical for tissue engineering and feasibility of drug therapy for strabismus.

OBJECTIVE: To explore an experimental method for primary culture of kitten extraocular muscle myoblast.

METHODS: Myoblasts from kitten extraocular muscle were obtained by the two-step enzymatic digestion method and pre-plating technique, and then passaged following primary culture in vitro. The cells were counted and observed by light microscopy combined with immunocytochemical identification.

RESULTS AND CONCLUSION: Myoblasts grew well under the growth medium and differentiated well under the differential medium, which could be fused into myotube and express Desmin. The two-step enzymatic digestion method conveniently permits the purification and proliferation of myoblasts. These results provide the basis for future studies to further observe the growth characteristics of myoblasts and discuss the feasibility of drug treatment of strabismus.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: strabismus, myoblasts, desmin, muscle contraction

CLC Number:

R318

Liu Tao, Liu Gui-xiang, Yin Ya-nan, Zhou Juan. Primary culture and identification of kitten extraocular muscle myoblasts[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8206-8211.

Figures/Tables 4

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