Construction of tissue engineered adipose using human adipose stem cells with chitosan-modified silk fibroin

doi:10.3969/j.issn.2095-4344.2014.39.016

Abstract

Abstract:

BACKGROUND: Based on the original advantages of silk fibroin, positive charged water-soluble chitosan modified silk fibroin is modified on surface and could improve cell adhesion on the scaffolds.
OBJECTIVE: To verify the biocompatibility of chitosan-modified silk fibroin with human adipose-derived stem cells (hADSCs), and feasibility of constructing tissue engineered adipose in vitro.
METHODS: The hADSCs at passage 3 were seeded on chitosan-modified silk fibroin at the concentration of 1×10 ⁷/L, as the experiment group; at the same cell concentration, hADSCs were seeded in 96-well plates as the control group. MTT tests were performed to evaluate the adhesion, growth and proliferation of hADSCs on chitosan-modified silk fibroin. Then hADSCs were implanted on the chitosan-modified silk fibroin scaffolds at the concentration of 1×10 ⁹/L. The hADSCs seeded onto chitosan-modified silk fibroin complexes were respectively cultured with adipogenic differentiation medium and ordinary high-glucose DMEM. The complexes were stained with oil red O, and detected with RT-PCR after cultured 14 days.
RESULTS AND CONCLUSION: The hADSCs adhered to and proliferated on the scaffolds. After cultured with adipogenic differentiation medium for 14 days, oil red O staining demonstrated that there were amount of mature adipocytes on the scaffold. The peroxisome proliferator activated receptor γ2 was positively expressed. The chitosan- modified silk fibroin possessed excellent biocompatibility in vitro. The co-cultured hADSCs could be induced to mature adipocytes successfully.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: chitosan, silk fibroin, mesenchymal stem cells

CLC Number:

R318

Kang Ting, Wang Gang, Liu Yi, Liu Gang-qiang . Construction of tissue engineered adipose using human adipose stem cells with chitosan-modified silk fibroin[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(39): 6323-6328.

Figures/Tables 7

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