Allogeneic platelet-rich plasma promotes wound collagen synthesis in diabetic rats

doi:10.3969/j.issn.2095-4344.2014.39.017

Abstract

Abstract:

BACKGROUND: Platelet-rich plasma has benefit in diabetic wound healing; however, the effect of allogeneic platelet-rich plasma is still unclear.
OBJECTIVE: To observe the effect of allogeneic platelet-rich plasma on collagen synthesis during diabetic wound healing.
METHODS: Streptozocin-induced diabetic rats were randomly divided into allogeneic platelet-rich plasma group and saline control group. Each group had 15 rats. A 1 cm2 full-thickness skin defect on the rat dorsum was excised. Platelet-rich plasma or saline was applied. Platelet-rich plasma was prepared from the whole blood of allogeneic healthy rats. Animals of each group were sacrificed on 3, 7, 14 days post operation. The differences of the wound closure rate, morphological character, hydroxyproline content and the relative mRNA expression of the collagen were observed.
RESULTS AND CONCLUSION: The wound closure rates were lower in control group on day 3, 7, 14 post operation (P < 0.05). Masson staining showed a decreased, disorder, loose collagen fibers distribution in diabetic control wound tissue. The results of the hydroxyproline test showed hydroxyproline content was significantly higher in platelet-rich plasma group at each time point (P < 0.01). The relative mRNA expression of type I and III collagen presented a higher expression in platelet-rich plasma treated wound tissue at each time point (P < 0.05). And the ratio between type I and III collagen was higher in platelet-rich plasma group at each time point (P < 0.05). In summary, allogeneic platelet-rich plasma can promote diabetic wounds healing, which may attribute to an enhanced collagen synthesis.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: platelet-rich plasma, collagen, wound healing, diabetes mellitus

CLC Number:

R318

Liu Chen, Zhang Hong-wei, Xu Ning. Allogeneic platelet-rich plasma promotes wound collagen synthesis in diabetic rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(39): 6329-6334.

Figures/Tables 6

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