Effect of a new-type enriched material on enriching bone marrow stem cells by selective cell retention

doi:10.3969/j.issn.2095-4344.2014.39.015

Abstract

Abstract:

BACKGROUND: Enriching material for bone marrow stem cells is a current research hotspot. Preparation and screening of a new type of bone marrow stem cells-enriched material is crucial for the development of tissue engineering and clinical applications.
OBJECTIVE: To observe the surface character and effect of a new type of bone marrow stem cells-enriched material on enriching bone marrow stem cells of decalcified bone matrix decorated with poly-L-lysine by selective cell retention technology.
METHODS: Decalcified bone matrix was decorated with poly-L-lysine as the scaffold material. Bone marrow mesenchymal stem cells were collected by using selective cell retention technology. Control group was decalcified bone matrix and experimental groups were Decalcified bone matrix decorated with poly-L-lysine at different concentrations (0.01%, 0.05%, 0.1%, 0.5%, 1%) with freeze-drying method. Material components were analyzed by Raman spectrum and infrared spectrum. Pore size, porosity, surface and cross section were observed under three-dimension video microscope and scanning electron microscope. The number of bone marrow nucleated cells, fibroblast colony-forming units and platelets were counted pre- and post-enrichment.
RESULTS AND CONCLUSION: Decalcified bone matrices were covered with well-distributed and dense poly-L-lysine. The poly-L-lysine/decalcified bone matrix had a high porosity with much more internally connected pores. After enrichment with enriched material prepared by 0.1% poly-L-lysine, the number of bone marrow nucleated cells, fibroblast colony-forming units and platelets were increased (3.18±0.31), (5.25±1.40) and (3.88±0.68) times, respectively; the binding efficiency were (53±12)%, (73±13)% and (34±10)%, respectively; the selective rate of fibroblast colony-forming units was 1.41±0.34. The new constructed poly-L-lysine/decalcified bone matrix has good three-dimension space with high selective retention. It is an effective enriching material for bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: tissue engineering, lysine, bone matrix, stents

CLC Number:

R318

Xu Zhen-dong, Wang Guo-dong, Liu Xi-ming, Cai Xian-hua, Xu Jian-zhong. Effect of a new-type enriched material on enriching bone marrow stem cells by selective cell retention[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(39): 6316-6322.

Figures/Tables 2

References

[1]Malizos KN, Dailiana ZH, Innocenti M, et al. Vascularized bone grafts for upper limb reconstruction: defects at the distal radius, wrist, and hand. J Hand Surg Am. 2010;35(10):1710- 1718.
[2]Giannoudis PV, Chris-Arts JJ, Schmidmaier G, et al. What should be the characteristics of the ideal bone graft substitute? Injury. 2011;42(Suppl 2):S1-S2.
[3]Block JE. The role and effectiveness of bone marrow in osseous regeneration. Med Hypotheses. 2005;65(4):740-747.
[4]蓝旭,文益民,葛宝丰,等.骨髓基质干细胞复合纳米羟基磷灰石/聚乳酸修复节段性骨缺损[J].中国骨与关节损伤杂志, 2006, 21(10):797-800.
[5]Lee K, Goodman SB. Cell therapy for secondary osteonecrosis of the femoral condyles using the Cellect DBM System: a preliminary report. J Arthroplasty. 2009;24(1):43-48.
[6]孙伟,赵杰,郭开今,等.富集骨髓间充质干细胞用于兔腰椎横突间的融合[J].中国组织工程研究,2012,16(10):1752-1755.
[7]胡德新,朱博,应小樟,等.含自体富集骨髓干细胞松质骨移植修复兔关节软骨大面积缺损的实验研究[J].浙江中西医结合杂志, 2014,24(3):200-202.
[8]应小樟,石仕元,胡德新,等.自体骨软骨移植与含富集骨髓干细胞松质骨移植修复兔关节软骨缺损[J].中国中西医结合外科杂志, 2010,16(6):674-677.
[9]Mahmood TA, Shastri VP, Van Blitterswijk CA, et al. Tissue engineering of bovine articular cartilage within porous poly (ether-ester) copolymer scaffolds with different structures. Tissue Eng. 2005;11(7-8):1244-1253.
[10]Muschler GF, Matsukura Y, Nitto H, et al. Selective retention of bone marrow-derived cells to enhance spinal fusion. Clin Orthop Relat Res. 2005;(432):242-251.
[11]Maddox E, Zhan M, Mundy GR, et al. Optimizing human demineralized bone matrix for clinical application. Tissue Eng. 2000;6(4):441-448.
[12]刘曦明,许建中,陈庄洪,等.PLL-DBM支架材料富集骨髓有核细胞的效果评价[J].华南国防医学杂志,2008,22(2):12-14.
[13]刘曦明,许建中,陈庄洪,等.新型骨髓干细胞富集材料的制备及其表征研究[J].华南国防医学杂志,2008,22(2):9-11.
[14]Hernigou P, Poignard A, Manicom O, et al. The use of percutaneous autologous bone marrow transplantation in nonunion and avascular necrosis of bone. J Bone Joint Surg Br. 2005;87(7):896-902.
[15]李强,孙正义,严冬雪,等.脱钙骨基质材料的生物学表现:降解性能、孔隙率及其黏附性能特征[J].中国组织工程研究与临床康复,2007,11(31):6121-6124.
[16]冯万文,夏亚一,党跃修,等.同种异体脱钙骨基质的组织工程学特性[J].中国组织工程研究与临床康复,2010,14(51):9512-9516.
[17]Urist MR. Bone: formation by autoinduction. Science. 1965; 150(3698):893-899.
[18]Randolph MA, Anseth K, Yaremchuk MJ. Tissue engineering of cartilage. Clin Plast Surg. 2003;30(4):519-537.
[19]李志强,侯天勇,罗飞,等.自组装肽复合脱钙骨基质作为骨髓富集支架材料的实验研究[J].第三军医大学学报,2011,33(5): 441-444.
[20]徐蕴,肖琼,田铧,等.细胞外基质对大鼠MSCs生物学活性的影响[J].中国现代普通外科进展,2007,10(1):26-28.
[21]Gareia AJ, Reyes CD. Bio-adhesive surfaces to promote osteoblast differentiation and bone formation. J Dent Res. 2005;84(5):407-413.
[22]Qian L, Saltzman WM. Improving the expansion and neuronal differentiation of mesenchymal stem cells through culture surface modification. Biomaterials. 2004;25(7-8):1331-1337.
[23]Siwach RC, Sangwan SS, Singh R, et al. Role of percutaneous bone marrow grafting in delayed unions, non-unions and poor regenerates. Indian JM ed Sci. 2001; 55(6):326-336.
[24]刘瑞祥,陈琪,涂江虹,等.自体骨髓经皮注射移植治疗骨不连和骨延迟愈合的临床效果观察[J].实用临床医药杂志,2013,17(16): 104-105.
[25]唐昭惠,诸黎星,徐土炳,等.自体红骨髓注射治疗骨不连术后局灶性骨缺损[J].中国骨伤,2009,22(7)549-550.
[26]Connolly JF. Injectable bone marrow preparations to stimulate osteogenic repair. Clin Orthop Relat Res. 1995;(313):8-18.
[27]Brodke D, Pedrozo HA, Kaput TA, et al. Bone grafts prepared with selective cell retention technology heal canine segmental defects as effectively as autograft. J Orthop Res. 2006;24(5): 857-866.
[28]干耀恺,戴尅戎,张蒲,等.应用富集骨髓干细胞技术治疗骨缺损[J].中华骨科杂志,2006,26(11):721-727.
[29]Jacobsen K, Szczepanowski K, Al Zube L A, et al. The role of intraoperative bone marrow aspirate stem cell concentration as a bone grafting technique. Tech Foot Ankle Surg. 2008; 7(2): 84-89.
[30]Youssef J, Brodke D, Haynesworth S, et al. 98. Selective cell retention technology in spinal fusion. Spine. 2003;3(5 Suppl): 114-115.
[31]Al-Obaidi MM, Al-Bayaty FH, Al Batran R, et al. Protective effect of ellagic acid on healing alveolar bone after tooth extraction in rat-a histological and immunohistochemical study. Arch Oral Biol. 2014;59(9):987-999.
[32]Congiu T, Pazzaglia UE, Basso P, et al. Chemical etching in processing cortical bone specimens for scanning electron microscopy. Microsc Res Tech. 2014;77(9):653-660.
[33]Oest ME, Damron TA. Focal therapeutic irradiation induces an early transient increase in bone glycation. Radiat Res. 2014; 181(4):439-443.
[34]Dai L, He Z, Zhang X, et al. One-step repair for cartilage defects in a rabbit model: a technique combining the perforated decalcified cortical-cancellous bone matrix scaffold with microfracture. Am J Sports Med. 2014;42(3):583-591.
[35]Mack SA, Maltby KM, Hilton MJ. Demineralized murine skeletal histology. Methods Mol Biol. 2014;1130:165-183.
[36]Koistinen AP, Halmesmäki EP, Iivarinen JT, et al. Short-term exercise-induced improvements in bone properties are for the most part not maintained during aging in hamsters. Exp Gerontol. 2014;51:46-53.
[37]Fu WL, Zhou CY, Yu JK. A new source of mesenchymal stem cells for articular cartilage repair: MSCs derived from mobilized peripheral blood share similar biological characteristics in vitro and chondrogenesis in vivo as MSCs from bone marrow in a rabbit model. Am J Sports Med. 2014; 42(3):592-601.
[38]Yorgan T, Rendenbach C, Jeschke A, et al. Increased Col10a1 expression is not causative for the phenotype of Phex-deficient Hyp mice. Biochem Biophys Res Commun. 2013;442(3-4):209-213.
[39]Thambyah A, Broom ND. Further insight into the depth-dependent microstructural response of cartilage to compression using a channel indentation technique. Comput Math Methods Med. 2013;2013:358192.
[40]Li ST, Liu Y, Zhou Q, et al. A novel axial-stress bioreactor system combined with a substance exchanger for tissue engineering of 3D constructs. Tissue Eng Part C Methods. 2014;20(3):205-214.
[41]Bondarenko A, Angrisani N, Meyer-Lindenberg A, et al. Magnesium-based bone implants: immunohistochemical analysis of peri-implant osteogenesis by evaluation of osteopontin and osteocalcin expression. J Biomed Mater Res A. 2014;102(5):1449-1457.
[42]Ko CL, Chen WC, Chen JC, et al. Properties of osteoconductive biomaterials: calcium phosphate cement with different ratios of platelet-rich plasma as identifiers. Mater Sci Eng C Mater Biol Appl. 2013;33(6):3537-3544.
[43]Tang H, Wu B, Qin X, et al. Tissue engineering rib with the incorporation of biodegradable polymer cage and BMSCs/decalcified bone: an experimental study in a canine model. J Cardiothorac Surg. 2013;8:133.
[44]Krause M, Oheim R, Catala-Lehnen P, et al. Metaphyseal bone formation induced by a new injectable β-TCP-based bone substitute: a controlled study in rabbits. J Biomater Appl. 2014;28(6):859-868.
[45]Kimura Y, Kubo S, Koda H, et al. RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection--a technical report. Hear Res. 2013;302:26-31.