Role of interleukin-1 receptor-associated kinase 4 activity in flap ischemia-reperfusion injury

doi:10.3969/j.issn.2095-4344.2014.36.017

Abstract

Abstract:

BACKGROUND: Interleukin-1 receptor-associated kinase 4 activity-induced inflammations and infection have been extensively accepted. However, there was no report concerning its effects on flap ischemia-reperfusion injury.
OBJECTIVE: To explore the significance of interleukin-1 receptor-associated kinase 4 activity in flap ischemia-reperfusion injury.
METHODS: A total of 36 adult male Sprague-Dawley rats were randomized into sham-operated group (n=12), ischemia-reperfusion group (n=12) and interleukin-1 receptor-associated kinase 4 group (n=12). The models of right lower abdominal island flap ischemia-reperfusion injury were set up. Interleukin-1 receptor-associated kinase 4 group was intraperitoneally injected with 1 mL of interleukin-1 receptor-associated kinase 4 (100 μmol/L) before reperfusion. The flaps were collected at 1, 2, 4, and 6 hours after ischemia-reperfusion injury for histopathhological observation. At 1 hour after ischemia-reperfusion, protein expression of interleukin-1 receptor-associated kinase 4 was detected in flaps. The proportion of flap survival was calculated at 7 days after surgery.
RESULTS AND CONCLUSION: Histopathological observation demonstrated that compared with the ischemia-reperfusion injury group, neutrophil infiltration and edema was evidently improved, and the protein expression of interleukin-1 receptor-associated kinase 4 was gradually reduced in the interleukin-1 receptor-associated kinase 4 group. Flap survival proportions were respectively (51.70 ±7.62)% and (86.56±12.23)% in the ischemia-reperfusion injury group and interleukin-1 receptor-associated kinase 4 group at 7 days after surgery. There were significant differences in the flap survival proportion between the two groups (P < 0.01). These results showed that after flap ischemia-reperfusion injury, the inhibition of interleukin-1 receptor-associated kinase 4 activities could elevate the survival rate of transplanted flap.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: interleukin-1 receptor-associated kinases, surgical flaps, reperfusion injury, models, animal

CLC Number:

R318

Zheng Liang1, Wu Xiao-wei, Song Hai-chen, Jian Qi-chao, Liu Ying-ying, Zhao Xiang-yi, Zhang Li-ming . Role of interleukin-1 receptor-associated kinase 4 activity in flap ischemia-reperfusion injury[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(36): 5836-5841.

Figures/Tables 2

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