Effect of Mailuoning on an ischemia/reperfusion injury model in the system of simulated in vivo physiological environment for amputated limbs

doi:10.3969/j.issn.2095-4344.2014.36.015

Abstract

Abstract:

BACKGROUND: Reducing the ischemia-reperfusion injury in the system of simulated in vivo physiological environment for amputated limbs, can extend the preservation time and improve the replantation success rate of amputated limb.
OBJECTIVE: To investigate the effect of Mailuoning on the ischemia-reperfusion injury in the system of simulated in vivo physiological environment for amputated limbs.
METHODS: The left hind legs harvested from 18 healthy adult male Bama mini-pigs were randomly divided into three groups: cold storage group, blood perfusion group, Mailuoning combined blood perfusion group, with six pigs in each group. After the left hind legs of each pig in all groups were amputated and stored at room temperature for 3 hours, the amputated legs were placed at 4 ℃, perfused with blood, or perfused with Mailuoning combined blood. After 6 hours of perfusion, the morphology of amputated limbs was observed under transmission electron microscope. The mRNA levels of apoptosis proteins Caspase3 and inflammatory factor interleukin-1β were detected by real time fluorescent quantitative RT-PCR.
RESULTS AND CONCLUSION: Transmission electron microscopy results showed that, the muscle fibers in blood perfusion group and Mailuoning combined blood perfusion group arranged more orderly and were more complete than cold storage group, the swelling of mitochondria was lighter. In addition, the condition in Mailuoning combined blood perfusion group was better than in blood perfusion group. RT-PCR results showed that the expression of Caspase3 and interleukin-1β was increased in all groups; at the same time, the expression level in blood perfusion group and Mailuoning combined blood perfusion group was significantly lower than those in cold storage group. Mailuoning combined blood perfusion group had s lower expression than blood perfusion group. In the system of simulated in vivo physiological environment for amputated limbs, Mailuoning can significantly reduce the ischemia/reperfusion injury of skeletal muscle cells and prolong the preservation time of amputated limbs.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: drug, Chinese herbal, hindlimb, cryopreservation, reperfusion injury

CLC Number:

R318

Zhang Shao-chun, Qin Xin-yuan, Zuo You-wei, Gao Lei, Yin Ye-feng, Wang Jiang-ning. Effect of Mailuoning on an ischemia/reperfusion injury model in the system of simulated in vivo physiological environment for amputated limbs[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(36): 5825-5829.

Figures/Tables 2

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