Expression of serum tumor necrosis factor-аlpha and interleukin-1beta in a vascular dementia rat model undergoing total flavonoids of hippophae

doi:10.3969/j.issn.2095-4344.2014.36.014

Abstract

Abstract:

BACKGROUND: Total flavonoids of hippophae have different degrees of regulation effect on the immune system. However, there is no available evidence addressing the effect of total flavonoids of hippophae on vascular dementia inflammatory factor.
OBJECTIVE: To observe the effect of total flavonoids of hippophae on serum tumor necrosis factor-α and interleukin-1β levels in a rat model of vascular dementia, and to discuss its interventional mechanism for vascular dementia.
METHODS: A total of 72 Wistar rats were randomly divided into six groups: sham operation, model, control, high-dose, medium-dose, and low-dose of total flavonoids of hippophae groups. Except for the sham operation group, rats in the other five groups were applied to establish vascular dementia models using the improved “vascular occlusion” method. Sham operation group was only subjected to the isolation of bilateral common carotid artery, without ligation. At 1 day after modeling, rats in control group was intragastrically given Ginkgo biloba extracts 48 mg/kg•d, while rats in total flavonoids of hippophae groups were given 200, 100, 50 mg/kg•d of total flavonoids of hippophae. Model group and sham operation group received equal volume of saline. After 30 days of administration, rats were detected in Morris water maze. Serum tumor necrosis factor-α and interleukin-1β levels of rats in different groups were detected after intervention.
RESULTS AND CONCLUSION: Compared with sham operation group, the vascular dementia model rats had a disordered learning and memory ability, and increased levels of serum tumor necrosis factor-α and interleukin-1β (P < 0.05). After intervention of Ginkgo biloba extracts and total flavonoids of hippophae, the learning and memory abilities were improved, and serum tumor necrosis factor-α and interleukin-1β levels were decreased (P < 0.05). Total flavonoids of hippophae can reduce serum tumor necrosis factor-α and interleukin-1β levels in vascular dementia rats. This may be the main mechanism of treating vascular dementia.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: hippophae, flavones, dementia, vascular

CLC Number:

R318

Qiao Xiao-ming, Ren Shi-cun. Expression of serum tumor necrosis factor-аlpha and interleukin-1beta in a vascular dementia rat model undergoing total flavonoids of hippophae[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(36): 5819-5824.

Figures/Tables 3

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