Modified method of establishing a rat model of myocardial infarction

doi:10.3969/j.issn.2095-4344.2014.18.017

Abstract

Abstract:

BACKGROUND: An animal model of myocardial infarction is of great significance for the study of the pathogenesis, pathophysiology and evaluation of treatment methods of human coronary artery disease. Many researchers aim to improve survival rate and the success rate of model induction.
OBJECTIVE: To improve the method of making a rat model of myocardial infarction.
METHODS: One hundred Wistar rats were divided into model group (n=80) and sham-operated group (n=20). The rats were intraperitoneally anesthetized with 3% sodium pentobarbital. Tracheostomy was operated. An automatic breathing apparatus was utilized for assisted breathing. A left anterior thoracotomy was performed at the fourth intercostal space. The left anterior descending coronary artery was ligated. Sham-operated rats underwent similar surgery but without coronary artery ligation. The thoracic cavity was closed layer by layer. The breathing apparatus was removed and tracheal intubation was also removed. To prevent suffocation, the trachea and the neck incision were not closed.
RESULTS AND CONCLUSION: Heart morphology and hematoxylin-eosin staining demonstrated that the success rate of establishing myocardial infarction model was 98.6%. The survival rate of model group at 3 weeks after surgery was 88.75%. Totally nine rats died, including seven dead rats during operation and within 24 hours after operation, and two dead rats in later stage. The survival rate of the sham-operated group was 100%. We modified the method of establishing a rat model of myocardial infarction and improved the operation success rate and survival rate of rats, such as anesthesia method, longitudinal incision during tracheotomy, without closing the trachea after operation, and the method of opening and closing the chest.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: myocardial infarction, models, animal, rats, Wistar, survival rate

CLC Number:

R318

Zhao Chuan-yan, Wang Xin. Modified method of establishing a rat model of myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(18): 2886-2891.

Figures/Tables 3

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