Correlation between the expression of vascular endothelial growth factor and microvessel density in collagen-induced arthritis rats

doi:10.3969/j.issn.2095-4344.2014.07.012

Abstract

Abstract:

BACKGROUND: Vascular endothelial growth factor is found the strongest to promote angiogenesis, which show strong expression in synovial cells in rheumatoid arthritis.
OBJECTIVE: To evaluate the relation between the expression of vascular endothelial growth factor and microvessel density in rheumatoid arthritis.
METHODS: Collagen II and complete Freund’s adjuvant were used to induce experimental arthritis in Sprague-Dawley rats. Synovium expression of vascular endothelium growth factor and microvessel density were assayed at different time points using immunohistochemistry method, and changes in new vessels, vascular endothelial growth factor expression and arthritis index were observed along the time course. The correlations between vascular endothelial growth factor expression and number of new blood vessels as well as between arthritis index and vascular endothelial growth factor expression were studied with linear correlation analysis and rank correlation analysis, respectively.
RESULTS AND CONCLUSION: With the time lasting, new blood vessels in the synovium proliferated, the synovium was thickened, and the arthritis index rose gradually as well as vascular endothelial growth factor expression and number of new microvessels were on the rise. Vascular endothelial growth factor expression was
positively correlated with arthritis index (r=0.535, P < 0.05) and significantly positively correlated with number of new microvessels (r=0.860, P < 0.01). Vascular endothelial growth factor is involved in the course of synovium pannus response, and vascular endothelial growth factor and microvessel density may play an important role in rheumatoid arthritis pathogenesis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: arthritis, rheumatoid, collagen, vascular endothelial growth factors, synovial membrane, microvessels

CLC Number:

R318

Yang Bo, Liang Qing-hua, Wu Dan, Xiong Xin-gui . Correlation between the expression of vascular endothelial growth factor and microvessel density in collagen-induced arthritis rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 1051-1056.

Figures/Tables 4

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