Recombinant human epidermal growth factor and basic fibroblast growth factor promote the proliferation of human corneal epithelial cells

doi:10.3969/j.issn.2095-4344.2014.07.011

Abstract

Abstract:

BACKGROUND: The preparation of recombinant human epidermal growth factor (rhEGF) and basic fibroblast growth factor (bFGF) has been clinically used in the repair of ocular surface trauma. However, the concentration of these growth factors that achieve the maximal healing effect and the comparison of two kinds of growth factors on promoting wound healing are still controversial.
OBJECTIVE: To investigate the effect of rhEGF and bFGF on the cloning of human corneal epithelial cells.
METHODS: The human corneal epithelial cells cultured in vitro were interfered with rhEGF and bFGF under different concentrations. The proliferation of human corneal epithelial cells was detected using MTT assay after 3, 5, 7 days of growth factors treatment. Plate clone formation assay was applied to observe the morphology of cell clone and analyze clone formation rate of human corneal epithelial cells.
RESULTS and CONCLUSION: MTT value shows that 10 μg/L rhEGF and 10 μg/L bFGF on day 5 were the most powerful concentration. The clone formation rate of human corneal epithelial cells after treated with 10 μg/L rhEGF was higher than that with 10 μg/L bFGF (P = 0.02). The results confirmed that both rhEGF and bFGF could promote the proliferation and increase clone formation ability of human corneal epithelial cells. 10 μg/L rhEGF for 5 days achieves the best effect on promoting clone formation of human corneal epithelium cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: epidermal growth factor, fibroblast growth factor, corneal epithelium

CLC Number:

R318

Peng Yan-yang, Wu Wei, Zeng Li-na, Chen Xiao-hong, Lu Xiao-he . Recombinant human epidermal growth factor and basic fibroblast growth factor promote the proliferation of human corneal epithelial cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 1045-1050.

Figures/Tables 1

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