Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (7): 1039-1044.doi: 10.3969/j.issn.2095-4344.2014.07.010
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Zhang Hui1, Liu Lin2, Xue Wen2
Revised:
2013-12-01
Online:
2014-02-12
Published:
2014-02-12
Contact:
Liu Lin, Chief physician, Department of Orthopaedics, People’s Hospital of Gansu Province, Lanzhou 730000, Gansu Province, China
About author:
Zhang Hui, Master, Attending physician, Department of Orthopaedics, Second People’s Hospital of Gansu Province, Lanzhou 730000, Gansu Province, China
Supported by:
the Natural Science Foundation of Gansu Province, No. 2010GS04988
CLC Number:
Zhang Hui, Liu Lin, Xue Wen. Extracelluar matrix stimulates neurite outgrowth of dorsal root ganglion neurons differently depending on whether extracted from degenerated or normal intervertebral disk[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 1039-1044.
2.2 人椎间盘组织神经生长因子浓度测定结果 正常人腰椎间盘髓核中神经生长因子浓度为(16.86±0.01) ng/L,与退变组(33.78±0.03) ng/L 相比较,差异有非常显著性意义(P < 0.001)。而正常纤维环组织为(13.53±0.02) ng/L,与退变组(15.68±0.01) ng/L比较,差异有显著性意义 (P < 0.05),见表1。提示在椎间盘源性下腰痛患者腰椎间盘组织中,无论纤维环或髓核中神经生长因子表达水平明显高于正常椎间盘组织。 2.3 大鼠脊神经背根神经节感觉神经元轴突生长测定结果 见表2。退变组神经元轴突平均长度显著长于正常组 (P < 0.05)。退变组加抗神经生长因子β干预后神经元轴突平均长度显著长于正常组加抗神经生长因子β干预后神经元轴突平均长度(P < 0.001)。 2.4 大鼠脊神经背根神经节感觉神经细胞P物质水平测定结果 在正常组的257个细胞中Ellsa染色阳性者59个(约22.9%),在退变组的305个细胞中Ellsa染色阳性者约175个(约57.4%),在正常组加抗神经生长因子β干预的98个细胞中Ellsa染色阳性者约44个(约44.9%),在退变组加抗神经生长因子β干预的213个细胞中Ellsa染色阳性者约35个(约16.4%)。由此可以看出,在退变人椎间盘组织培养液中P物质比率明显高于正常组(P < 0.001),差异有非常显著性意义。"
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