Mechanism of traditional Chinese medicine Shixiang plaster in promoting the repair of soft tissue defects

doi:10.3969/j.issn.2095-4344.1384

Abstract

Abstract:

BACKGROUND: Shixiang plaster has been shown to be effective for treating soft tissue defects and chronic healing.
OBJECTIVE: To explore the mechanism of traditional Chinese medicine Shixiang plaster to promote the repair of soft tissue defects.
METHODS: Thirty adult male rabbits were provided by Laboratory Animal Center of Guizhou Medical University, and the study was approved by the Experimental Animal Ethics Committee of Guiyang University of
Traditional Chinese Medicine. All rabbits underwent classic full-thickness skin-cutting model, and then randomly divided into Shixiang plaster group (Shixiang plaster + dressing), Beifuji group (recombinant bovine basic fibroblast growth factor + dressing) and blank control group (dressing). Dressing change was performed at 3, 6, 9 and 12 days, respectively, and the wound healing and granulation tissue were observed, and the wound healing time was recorded. The specimens of newborn granulation tissue were collected to observe the number of fibroblasts and vascular changes by pathological section. The expression levels of basic fibroblast growth factor and transforming growth factor-β1 protein in wounds were detected.
RESULTS AND CONCLUSION: (1) The wound healing rate in the Shixiang plaster and Beifuji group was high, the healing time was short, and the curative effect was better than that in the blank control group. (2) Pathological observation: Shixiang plaster group showed obvious capillary proliferation, mild inflammatory reaction, many fibroblasts and inflammatory cell infiltration in new granulation. The expression levels of transforming growth factor-β1 and basic fibroblast growth factor protein in wounds at 6 and 12 days after modeling under the same conditions in all groups were significantly higher than those at 3 days after modeling (P < 0.05). (3) These results suggest that Shixiang plaster can increase the expression levels of endogenous growth factors transforming growth factor-β1 and basic fibroblast growth factor in wounds and promote the repair of soft tissue defects.

Key words: Shixiang plaster, soft tissue defect repair, transforming growth factor-β1, basic fibroblast growth factor, chronic healing wound

CLC Number:

Zhu Xu, Shen Jun, Yang Rongkun, Wang Yong, Liu Yang, Sun Quan, He Zhuying, Chen Jiuyi. Mechanism of traditional Chinese medicine Shixiang plaster in promoting the repair of soft tissue defects [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(27): 4350-4355.

Figures/Tables 1

2.1 实验动物数量分析实验选用新西兰大白30只，分为3组，实验过程无脱失，全部进入结果分析。 2.2 创面愈合时间、愈合率 2.2.1 创面愈合时间整体比较(单因素方差分析)得知：3组创面愈合时间整体差异有显著性意义(F=39.742，P < 0.05)。多重比较并结合数据分析：石香膏组和贝复济组的平均愈合时间分别为(14.51±0.79)d和(13.55±0.90)d，空白组为(18.25±1.79)d。石香膏组与空白对照组比较愈合时间明显缩短(P < 0.05)。 2.2.2 创面愈合率整体比较(两因素重复测量方差分析)得知：组间、时间及交互作用均有显著性意义(F组间= 201.712，P组间=0.000；F时间=1 984.686，P时间=0.000 P < 0.05)；资料球型性校正采用HF系数法；组间精细比较为LSD-t检验，两两精细比较并结合数据分析：从第6天起，石香膏组与贝复济组的创面愈合率，皆高于空白对照组 (P < 0.05)。见表1。 2.3 病理组织学观察 ①造模后第3天，3组创面组织炎症反应较明显，在邻近创伤的真皮中，有大量炎性细胞浸润；②造模后第6天，相比空白对照组，石香膏组和贝复济组上皮爬行较快，石香膏组创面脓液覆盖，肉芽组织鲜红，可见毛细血管增生，新生肉芽中可见许多成纤维细胞；贝复济组可见大量梭形的成纤维细胞增生，见胶原沉积；空白组能够观察到肉芽组织的生长，相比前2组，但再生毛细血管数量较少；③造模后第12天，大量的新生表皮覆盖在石香膏组和贝复济组创面上，肉芽组织内的毛细血管渐闭合，退化。而在空白组中，能够发现进一步增厚的新生表皮。见图1。 2.4 三组创面不同时期转化生长因子β1表达比较见表2。经两因素重复测量方差分析：其整体组间、时间及交互作用差异均有显著性意义(F组间=18.574，P组间=0.000； F时间=500.339，P时间=0.000)。资料球型性校正采用HF系数法。组间精细比较为LSD-t 检验，两两精细比较并结合数据分析：①造模后3 d：3组转化生长因子β1均有阳性表达，3组间差异无显著性意义(P > 0.05)；②造模后6 d：3组转化生长因子β1阳性表达均升高，相比空白组，石香膏组和贝复济组A值明显的升高(P < 0.05)，而石香膏组和贝复济组间差异无显著性意义(P > 0.05)；③造模后12 d：相比前几日，3组转化生长因子β1阳性表达均有升高，升高最为明显的为空白对照组，对3组的A值进行两两比较发现，差异均有显著性意义(P < 0.05)。见图2。 2.5 三组创面不同时期碱性成纤维细胞生长因子表达比较见表3。经两因素重复测量方差分析：其整体组间、时间及交互作用均有显著性意义(F组间=478.287，P组间=0.000；F时间=3 455.181，P时间=0.000 P < 0.05)。资料球型性校正采用HF系数法。组间精细比较为LSD-t 检验，两两精细比较并结合数据分析：①造模后3 d，3组创面新生肉芽组织及创缘皮肤中碱性成纤维细胞生长因子均有阳性表达，其中石香膏组和贝复济组高于空白组(P < 0.05)，存在显著性差异；②造模后6 d，3组创面新生肉芽组织及创缘皮肤内，碱性成纤维细胞生长因子阳性表达A值均升高，而相比空白组，石香膏组和贝复济组升高明显(P < 0.05)，石香膏组和贝复济组组间比较，无显著性差异；③造模后12 d，3组创面的创缘皮肤和新生肉芽组织内的碱性成纤维细胞生长因子阳性表达A值都升高，相比贝复济组，石香膏组和空白组升高更明显(P < 0.05)，石香膏组和空白组组间比较，无显著性意义。见图3。 "

References

[1]付小兵,程天民,陆树良,等.中国人体表难愈合创面发生新特征与防治的创新理论与关键措施研究[Z].中国人民解放军总医院第一附属医院,中国人民解放军第三军医大学,上海交通大学医学院附属瑞金医院,温州医科大学,深圳普门科技有限公司,无锡市第三人民医院,中国人民解放军第三零六医院,上海交通大学医学院附属第九人民医院,苏州大学,中国人民解放军总医院. 2015.

[2]费冀,陈久毅,张开伟,等.中药石香膏促进慢性难愈合创面修复的随机对照临床研究[J].辽宁中医杂志,2015,42(2):236-238.

[3]朱旭,陈久毅,王勇.中药促进指端缺如再生修复临床初探[J],内蒙古中医药,2012,31(1):50-50.

[4]费冀,陈久毅.中药“石香膏”促进糖尿病溃疡创面修复临床初探[J].亚太传统医药,2014,10(3):107-108.

[5]van Solingen C, Araldi E, Chamorro-Jorganes A, et al. Improved repair of dermal wounds in mice lacking microRNA-155. J Cell Mol Med,2014,18(6):1104-1112.

[6]邓步华.病理检验技术[M].北京:高等教育出版社,2005:77-81.

[7]Rawat S,Singh R,Thakur P,et al.Wound healing Agents from Medicinal Plants: A Review. Asian Pacific Journal of Tropical Biomedicine.2012;2(3):S1910-S1917.

[8]朱梦茹,郭澍,刘洋,等.脂肪来源干细胞外泌体在组织修复和再生医学中的研究进展[J].中国美容整形外科杂志, 2018,29(12): 757-760.

[9]高小月,张玉泉,杨晓清.人脐带间充质干细胞在组织损伤修复中的研究进展[J].生物医学工程与临床,2018, 22(2):208-213.

[10]谢玉国,陈伟鹏,曾庆湖.旋髂浅动脉皮瓣修复手部皮肤软组织损伤中的治疗效[J].中国临床研究,2016,29(6):774-776.

[11]Eming SA, Martin P ,Tomic-Canic M.Wound repair and regeneration: Mechanisms, signaling,and translation. Sci Transl Med. 2014 Dec 3;6(265):265sr6.

[12]Reinke JM, Sorg H.Wound Repair and Regeneration. Eur Surg Res. 2012;49(1):35-43.

[13]Velnar T , Ba T , Smrkolj V . The Wound Healing Process: An Overview of the Cellular and Molecular Mechanisms. J Int Med Res. 2009;37(5):1528-1542.

[14]Buchanan PJ,Kung TA,Cederna PS.Evidence-based medicine: Wound closure.Plastic & Reconstructive Surgery. 2014;134(6):1391-1404.

[15]Sonnemann KJ, Bement WM. Wound Repair: Toward Understanding and Integration of Single-Cell and Multicellular Wound Responses. Annu Rev Cell Dev Biol. 2011;27: 237-263.

[16]Thangapazham RL, Sharad S, Maheshwari RK. Phytochemicals in Wound Healing. Adv Wound Care (New Rochelle). 2016;5(5):230-241.

[17]Barrientos S, Stojadinovic O , Golinko M S , et al. Growth factors and cytokines in wound healing. Wound Repair Regen. 2008;16(5):585-601.

[18]Shi HX,Lin C,Lin BB,et al.The antiscar effects of basic fibroblast growth factor on the wound repair in vitro and in vivo. PLoS One.2013;8(4):e59966.

[19]武姗,刘洋,朱旅云,等. 转化生长因子β及其受体在糖尿病足创面愈合过程中作用机制的研究进展[J]. 中国糖尿病杂志,2017, 25(6):569-572,

[20]周思政.皮肤创伤愈合和增生性瘢痕动物模型的研究进展[J].组织工程与重建外科杂志,2018,14(1):48-52.

[21]Yin H, Li XY,Hu SL,et al.IL-33 accelerates cutaneous wound healing involved in vedinupr upreguatlion ahernatively aivated macrophages.Molecular Immunology.2013; 56:347353.

[22]刘公洪.湿性敷料治疗在烧伤创面的应用进展[J]. 西南军医, 2012,14(1):113-114.

[23]武瑞仙,梁晨,王春晖,等.中医古籍中祛腐生肌方剂用药规律分析[J].辽宁中医杂志,2016,43(6):1146-1149.

[24]张晓芬,孙桂芳,陈亚峰,等.活血化瘀中药促进创面愈合的研究进展[J].光明中医,2015,30(8):1816-1820.

[25]聂元董,李刚.活血化瘀中药治疗足踝部软组织缺损皮瓣移植术后组织修复的临床研究[J].临床医药文献电子杂志, 2016,3(51): 10221.