Effects of warming the yang and benefiting the marrow on the expression of matrix metalloproteinases in articular cartilage of rabbits with knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2014.07.003

Abstract

Abstract:

BACKGROUND: There are few reports concerning effects of warming the yang and benefiting the marrow for the knee osteoarthritis on the expression of matrix metalloproteinase.
OBJECTIVE: To observe the effect of warming the yang and benefiting the marrow on the expression of matrix metalloproteinase in rabbit models of knee osteoarthritis.
METHODS: Of 96 healthy adult New Zealand rabbits, 72 rabbits were randomly selected for making rabbit models of knee osteoarthritis using plaster external fixation. After success model establishment, the rabbits were randomly assigned to three groups. Model group was left intact. Chinese medicine group received daily intragastric administration of drug extract 24 mL/kg. Drug control group was daily intragastrically administered Puli Capsule (glucosamine hydrochloride) 24 mg/kg, once a day, until the eighth week of success model induction. An additional 24 New Zealand rabbits served as blank controls.
RESULTS AND CONCLUSION: Using quantitative PCR, matrix metalloproteinase-1, matrix metalloproteinase-3 and matrix metalloproteinase-13 expression was significantly higher in the model group than that in the other three groups. Matrix metalloproteinase-1, matrix metalloproteinase-3 and matrix metalloproteinase-13 expression was significantly lower in the Chinese medicine group and drug control group than that in the model group. These results indicated that warming the yang and benefiting the marrow for knee osteoarthritis in rabbits could effectively inhibit the expression of matrix metalloproteinase in rabbits.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: osteoarthritis, cartilage, drugs, Chinese herbal, matrix metalloproteinase

CLC Number:

R318

Shan Peng-cheng, He Ming-jiang, Zhang Hong-mei, Jing Lin, Zhang Chun, Zhang Ren-zhuo, Yan Qi, Sha Yi-hui. Effects of warming the yang and benefiting the marrow on the expression of matrix metalloproteinases in articular cartilage of rabbits with knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 997-1002.

Figures/Tables 2

References

[1] Willian Hunter.Of the structure and disease of the articular cartilage .Clin Orthop.2006;11(3):317.

[2] 金晓飞,郭长青,蒋昭霞,等. 膝骨关节炎模型内侧副韧带的生物力学特性[J]. 中国组织工程研究与临床康复,2011,15(43): 8046-8049.

[3] 张尚普,史晨辉,赵瑾. 强力霉素对兔制动骨关节炎模型的治疗作用[J].中国矫形外科杂志,2009,17(12): 935-937.

[4] Mort JS Billington CJ. Articular cartilage and changes in arthritis matrix degradation. Arthritis Res.2001;3:337-341.

[5] Shiozawa S, Tsumiyama K. Pathogenesis of rheumatoid arthritis and c-Fos/AP-1. Cell Cycle. 2009;8(10):1539-1543.

[6] Scarpellini M, Lurati A, Vignati G, et al. Biomarkers, type II collagen, glucosamine and chondroitin sulfate in osteoarthritis follow-up: the Magenta osteoarthritis study. J Orthop Traumatol. 2008;9(2):81-87.

[7] 林剑,王胜,刘铮.加味四妙散对膝骨关节炎患者MMPs-1、TIMPs-1的影响[J].中国中医药科技,2013,20(4): 336-337.

[8] 李守龙,艾依热提•买买提,潘海涛,等.膝骨关节炎滑膜不同部位基质金属蛋白酶3的表达及临床意义[J].首都医科大学学报, 2013, 34(3): 411-415.

[9] 吴琪,任婕,胡华,等.徐长卿丹皮酚对兔膝骨性关节炎关节软骨MMP-1和TIMP-1表达的研究[J].湖北中医药大学学报, 2013, 15(3):9-12.

[10] 李萍,马燕红.基质金属蛋白酶在骨关节炎中的作用[J]. 中国康复,2013,28(3):218-222.

[11] 董刚,乐军,周辉,等. PDTC关节腔注射对兔创伤性OA软骨MMP-1、MMP-9 mRNA表达的影响[J].浙江中西医结合杂志, 2013,(8): 609-612.

[12] 李俊,刘日光,邱波,等.血管内皮生长因子对关节软骨细胞mmP-1及mmP-3表达的影响[J].中国医药导刊,2013,(3): 519-521.

[13] 左炳光,薄海,彭朋. 持续被动运动对兔骨关节炎模型软骨细胞iNOS、MMP-1表达及NO含量的影响[J].现代中西医结合杂志, 2013,22(10): 1049-1050,1140.

[14] 侯小丽,赵林涛.金天格胶囊对骨关节炎大鼠MMP-3和TIMP-1的影响[J]. 河南中医,2013,33(3): 356-358.

[15] 刘荣清,林佳静,孙伯坚,等.类风湿关节炎和骨关节炎滑膜滑液中MMP-2和MMP-9的比较[J].宁夏医科大学学报,2013,35(1): 23-25.

[16] 李卫平,胥方元,蹇睿,等.电针对实验性兔膝骨关节炎模型白细胞介素-1β和基质金属蛋白酶-1表达的影响[J].中国康复医学杂志, 2013,28(2): 139-142.

[17] 陈琼,赵明才,陈悦,等.姜黄素对骨关节炎软骨细胞增殖及分泌MMP-13,IL-6的影响[J].现代中西医结合杂志,2013,22(5): 459-461.

[18] 侯亚平,陈太金. 骨痹汤对膝骨关节炎患者滑液中MMP-13、IL-1、TNF-α、TIMP-1的影响[J].世界中医药,2012,7(6):488-490.

[19] 徐华明,孟祥奇,姜宏,等.大鼠中药血清对骨关节炎患者膝关节软骨细胞胶原和金属蛋白酶mRNA表达的影响[J].江苏中医药, 2012, 44(12): 75-77.

[20] 孙书龙,姜宏,汤晓晨,等.黄芪甲苷对人膝骨关节炎退变关节软骨细胞基质金属蛋白酶-1及基质金属蛋白酶-3mRNA表达的影响[J].中医正骨,2012,24(10): 5-9.

[21] 袁琴,阚卫兵,宋朋飞,等.补肾活血方对大鼠膝骨关节炎滑膜细胞β-catenin、MMP-7的影响[J].中国骨伤,2012,25(9):761-765.

[22] 张金山,程园园,刘健.膝骨关节炎患者血清MMP-3、TIMP-1水平变化及相关性研究[J].安徽医科大学学报,2012,47(9):1066- 1069.

[23] 张恩水,闫新峰,张明,等. 聚蛋白多糖酶和基质金属蛋白酶在骨关节炎不同时期关节液中的表达[J]. 山东大学学报:医学版, 2012, 50(7): 87-91.

[24] 李卫平,蹇睿,胥方元. 超声波治疗兔膝骨关节炎对MMP-1和MMP-13表达的影响[J]. 重庆医学,2012,41(16): 564-1566.

[25] Stanton H, Rogerson FM, East CJ, et al. ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro. Nature 2005;434: 648-652.

[26] Green DM, Noble PC, Bocell JR Jr, et al. Effect of early full weight-bearing after joint injury on inflammation and cartilage degradation. J Bone Joint Surg Am. 2006;88:2201-2209 .

[27] Takahashi M, Naito K, Abe M, et al. Relationship between radiographic grading of osteoarthritis and the biochemical markers for arthritis in knee osteoarthritis. Arthritis Res Ther 2004;6:R208-R212.

[28] Little CB, Barai A, Burkhardt D, et al. Matrix metalloproteinase 13–deficient mice are resistant to osteoarthritic cartilage erosion but not chondrocyte hypertrophy or osteophyte development. Arthritis Rheum.2009;60:3723-3733.

[29] 郭秦炜,田得祥,敖英芳.骨性关节炎关节软骨中I型、II型、III型及X型胶原的分布[J]. 中国运动医学杂志, 2002, 21(3):228-231.

[30] Johnson AR, Pavlovsky AG, Ortwine DF, et al. Discovery and characterization of a novel inhibitor of matrix metalloprotease-13 that reduces cartilage damage in vivo without joint fibroplasia side effects. J Biol Chem ,2007;282: 27781-91.

[31] Liao EY, Luo XH. Effects of 17beta-estradiol on the expression of matrix metalloproteinase-1, -2 and tissue inhibitor of metalloproteinase-1 in human osteoblast-like cell cultures.Endocrine. 2001;15(3):291-295.

[32] Mahmoud RK,EI-Ansary AK,El-Eishi HH,et al.Matrix metalloproteinases MMP-3 and MMP-1 levels in sera and synovial fluids in patients with rheumatoid arthritis and osteoarthritis.Biochem.2005;54:248-257.

[33] Liacini A,Sylvester J,Zafarullah M,et al.Mithramycin downregulates proinflammatory cytokine-induced matrixmetallop roteinase gene expression in articular chondrocytes.Arthritis Res Ther.2005;7:777-783.

[34] Reboul P,Pelletier JP,Tardif G,et al.The new collagenase,collagenase-3,is expressed and synoviocytes.A role in osteoarthritis.J Clin Invest.1996;97: 2011-2019.

[35] 王玉彬,陈安民,郭风劲,等.基质金属蛋白酶家族在骨关节炎软骨组织中表达的研究[J].中国矫形外科杂志,2007,11(15):853-855.

[36] Dahlberg L,Billinghurst RC,Manner P,et al.Selective enhancement of collagenase-mediated cleavage of resident type Ⅱ collagen in cultured osteoarthritic cartilage and arrest with a synthetic inhibitor that spares collagenase 1( matrix metalloproteinase 1).Arthritis Rheum.2000;43:673-682.

[37] Boris VS,Gerald NS.Differential patterns of response to doxcycline and tansforming growth factor β1 in the down-regulation of collagenases in osteoarthritic and normal human chondrocytes .Arthritis Rheumatism.1999;4:719-727.

[38] 王毅,刘长明.基质金属蛋白酶与骨关节疾病关系的研究进[J].中国骨伤,2003,16: 190-192.

[39] Jain A, Nanchahal J, Troeberg L, et al. Production of cytokines,vascular endothelial growth factor, matrix metalloproteinases, and tissue inhibitors of metalloproteinases 1 by tenosynovium demonstrates its potential for tendon destruction in rheumatoid arthritis. Arthritis Rheum.2001;44: 1754- 1760.

[40] Kubota T, Kubota E, Matsumoto A, et al. Identification of matrix metalloproteinases (MMPs) in synovial fluid from patients with temporomandibular disorder. Eur J Oral Sci. 1998;106: 992- 998.

[41] Ishiguro N, Ito T, Ito H, et al. Relationship of matrix matalloproteinases and their inhibitors to cartilage proteoglycan and collagen turnover; analysis of synovial fluid from patients with osteoarthritis.Arthritis Rheum.1999;42: 129-136.

[42] Flannery CR, Lit tle CB, Cat erson B, et al. Ef fects of culture conditions and exposure to catabolic stimulators (IL-1 and retinoic acid) on the expression of matrix metalloproteinases (MMPs) and disintegrin metalloproteinases (ADAMs) by articular cartilage chondrocytes. Matrix Biol.1999;18(3) : 225 -237.

[43] Naito K, Takahashi M, Kushida K, et al. Measurement of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases-1(TIMP-1)in patients with knee osteoarthritis: comparision with generalized osteoarthritis. Rheumatology (Oxford).1999;38: 510- 554.

[44] 王国金,卫小春,纪斌平,等. 血清和关节液中基质金属蛋白酶-3及其抑制因子-1的检测及临床意义[J]. 中华风湿病学杂志, 2006,5(10):311-313.

[45] 李彦林,王国梁,曹斌,等. AMD3100体外阻断基质细胞衍生因子1/趋化因子受体4信号通路对人关节软骨细胞分泌基质金属蛋白酶3、9、13水平的影响[J].中国修复重建外科杂志,2012,26(6): 652-656.

[46] 伍捷进,吴启富,接红宇,等. 健骨颗粒冲剂对膝骨关节炎大鼠血清和关节液中肿瘤坏死因子-α及基质金属蛋白酶-3的影响[J]. 中医正骨,2012,24(5):20-22.

[47] 赵敬河,杨秋云,王冰.骨关节炎时内源性细胞因子及基质金属蛋白酶抑制因子1对关节软骨的影响[J].中国组织工程研究,2012, 16(15): 2665-2668.

[48] 李彦林,王国梁,曹斌,等. 玻璃酸钠与膝骨关节炎患者关节液中基质细胞衍生因子1和基质金属蛋白酶3,9,13水平的相关性[J]. 中国组织工程研究,2012,16(7):1323-1326.

[49] 李石胜,吴耀持,张峻峰,等. 长针透刺膝骨关节炎模型大鼠滑膜组织中基质金属蛋白酶3的变化[J]. 中国组织工程研究与临床康复,2011,15(50): 9415-9418.

[50] 方锐,刘振锋,艾力江•阿斯拉,等. 维药买朱尼含药血清影响大鼠关节软骨细胞基质金属蛋白酶1及其组织抑制因子1的表达[J]. 中国组织工程研究与临床康复,2011,15(50):9327-9330.

[51] 于浩淼,郭艾,杨林.基质金属蛋白酶13抑制剂在骨关节炎治疗中的现状与展望[J].中华关节外科杂志:电子版,2011,5(6): 774-780.

[52] 陈益丹,邱华平,金肖青,等.温针灸对兔膝骨关节炎模型基质金属蛋白酶及组织形态学影响的实验研究[J].中华中医药学刊,2011, 29(5):1060-1062.