Large vascularized tissue-engineered bone for repair of bone defects

doi:10.3969/j.issn.2095-4344.2014.07.002

Abstract

Abstract:

BACKGROUND: Currently, there are many problems existing in the construction of large tissue-engineered bone, and the most important is ischemic necrosis. Vascularization is critical for the biological function of large tissue-engineered bone.
OBJECTIVE: To prepare large pieces of vascularized tissue-engineered bone for orthotopic transplantation in repairing large femoral defects of rabbits, and to explore tissue regeneration methods and characteristics.
METHODS: Rabbit femoral structure was firstly imitated to construct a vascularized prefabricated model, based on which, we constructed large pieces of vascularized tissue-engineered bone. A total of 18 New Zealand white rabbit 18, aged 8 weeks, were divided into experimental group and control group. The experimental group was subject to orthotopic transplantation of large vascularized tissue-engineered bone, while the control group was treated with orthotopic transplantation of large tissue-engineered bone without vascularization processing. After 2, 4, 8 weeks, gross observation, X-ray films, and tissue section were compared between the two groups.
RESULTS AND CONCLUSION: Gross observation and imaging observation showed that the osteogenesis in the experimental group at 2, 4, 8 weeks were better than that in the control group. The ratio of new bone tissue to total area of bone graft was significantly higher in the experimental group than the control group at 2, 4, 8 weeks postoperatively (P < 0.01). These findings indicate that large vascularized tissue-engineered bone is preferred to the repair of large bone defects.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: tissue engineering, osteonecrosis, mesenchymal stem cells, osteoblasts, neovascularization, physiologic

CLC Number:

R318

Li Yao-hua. Large vascularized tissue-engineered bone for repair of bone defects[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 991-996.

Figures/Tables 1

2.1 实验动物数量分析造模未造成动物死亡，进入结果分析最终为18只兔，无脱失，实验组和对照组各9只。 2.2 大体观察造模后2周时，实验组新生组织软骨层内充满不成熟软骨样组织，胶原纤维杂乱排列，可以观察到明显的纤维结缔组织连接；对照组仅有少量骨痂形成。造模后4周，实验组有大量连续性骨痂形成，并有血管长入支架内，且血管周围形成编织骨；对照组仅有骨痂形成较前有所增多。造模后8周，实验组大块血管化组织工程骨直径和厚度基本和宿主骨相同，其中有2只比宿主骨还要厚；对照组有大骨痂形成，但是仍然骨不连。 2.3 影像学检查造模后2周时，实验组X射线摄片大块血管化组织工程骨处可见大片云雾状密度增高影；对照组仅显支架上下端有少许云雾状密度增高影。造模后4周，实验组有X射线摄片可见大块血管化组织工程骨周围有片状阻射影，骨连接处界线模糊；对照组云雾状密度增高影有所增多，但是骨连接处界线清晰。造模后8周，实验组X射线摄片可见大块血管化组织工程骨显骨性连续性连接，为连续性高密度影；对照组有大片状密度增高影，骨连接处界线仍然模糊可见。 2.4 组织学检查造模后2周时，实验组可见大量毛细血管增生周边可见新生骨小梁，大块血管化组织工程骨周围有丰富的纤维组织；对照毛细血管少，且较短小未能长入支架中，新生骨小梁也明显少于实验组，支架周围纤维组织也少。造模后4周，实验组工程骨周围血管丰富，基本布满整个工程骨，纤维组织下有大量新生骨组织，并可见大量活跃的成骨细胞；对照毛细血管较前增多，周围有少量新骨形成。造模后8周，实验组的大块血管化组织工程骨已经和宿主骨被骨组织桥接，并逐渐形成板成骨内有髓腔样结构；对照组也有较多成骨细胞核新生骨，但是和实验组比较差别明显。 2.5 新生骨组织占总移植骨的面积比通过对比造模后2，4，8周新生骨组织占总移植骨面积比，2组之间差异有显著性意义(P < 0.01，表1)。"

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