Cartilage surface of early osteoarthritis in rats induced by papain under scanning electron microscope

doi:10.3969/j.issn.2095-4344.2014.02.003

Abstract

Abstract:

BACKGROUND: Osteoarthritis animal models induced by papain are commonly used for studying osteoarthritis.
OBJECTIVE: To observe the morphology of articular cartilage surface during early osteoarthritis process in rats induced by papain and L-cysteine under scanning electron microscope.
METHODS: The right knees of Sprague-Dawley rats were given 0.15 mL mixed solution of 2% papain and 0.03 mol/L L-cysteine in the ratio of 2:1. The left knees were given injections of 0.15 mL 0.9% NaCl, as the control group. Another two rats (four knees) maintained untreated, as the normal control group. All the rats were observed with scanning electron microscope at 2, 4, 6 weeks after injection, to explore the surface morphology of medial condyle of femur.
RESULTS AND CONCLUSION: The condyles of normal and control groups revealed numerous shallow pits on the surface of articular cartilage. At 2 weeks after injection, the surfaces of condyles were unsmooth, presenting the shrinkage and distortion. The thinner articular cartilage and local tiny cracks were seen at 4 weeks. The deep and great cracks and cartilage defect were observed on the surface of cartilage at 6 weeks. The time of 4- 6 weeks is a reference point for establishing early osteoarthritis model in rats through injections of 2% papain and 0.03 mol/L L-cysteine.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: osteoarthritis, papain, L-cysteine, cartilage, articular

CLC Number:

R318

Wang Zong-bao, Xu Ya-lin, Liao Wei-ming, Lu Qing-you, Chen Zhao-hui, Wu Xiang-yang, Sun Xu, Duan Wen-xiu, Bao Yong-jun, Dai Yue-lan. Cartilage surface of early osteoarthritis in rats induced by papain under scanning electron microscope[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(2): 177-182.

Figures/Tables 1

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