Insulin-like growth factor 1 promotes in vitro proliferation of chondrocytes in traumatic arthritis

doi:10.3969/j.issn.2095-4344.2014.02.004

Abstract

Abstract:

BACKGROUND: The early damaged chondrocytes are susceptible to de-differentiate and exert unstable phenotype during the in vitro culture, thus needing some growth factors.
OBJECTIVE: To observe the promotion effect of insulin-like growth factor 1 on the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis.
METHODS: Traumatic arthritis models of adult rabbits were established by using the modified Hulth method. After the models were successfully established, the distal femur and proximal tibia were harvested under sterile conditions, the chondrocytes were cultured. The cultured cells were divided into two groups: control group was cultured with Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, while experimental group was cultured with Dulbecco's modified Eagle’s medium containing 100 μg/L insulin-like growth factor 1. The effect of insulin-like growth factor 1 on the proliferation of chondrocytes in adult rabbits with traumatic arthritis was determined through the cytomorphology, cell counting, and cell activity.
RESULTS AND CONCLUSION: The chondrocytes in adult rabbits with traumatic arthritis were successfully cultured, the majority of cells were mini-cells, presenting small fusiform, round or polygonal shape. Hematoxylin-eosin staining showed that the number of cells in experimental group was higher than that in control group. MTT assay found that the absorbance of cells in experimental group was greater than that in control group (P < 0.01). Our findings indicate that, insulin-like growth factor 1 can promote the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: insulin-like growth factor I, arthritis, model, animals, chondrocytes, cell proliferation

CLC Number:

R318

Zhao Guang, Jing Ji-feng, Zhang Zhi-yu, Zhu Jia-jun, Cui Yan. Insulin-like growth factor 1 promotes in vitro proliferation of chondrocytes in traumatic arthritis[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(2): 183-186.

Figures/Tables 2

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