Rhein and archen induce the apoptosis of mesangial cells in high glucose rats

doi:10.3969/j.issn.2095-4344.2013.44.014

Abstract

Abstract:

BACKGROUND: Our previous study has already manifested that Chinese medicine Xiaokening can effectively prevent and treat the early diabetic nephropathy.
OBJECTIVE: To compare the effect of rhein and archen on the apoptosis of mesangial cells of rats cultured with high glucose.
METHODS: Mesangial cells were stimulated by different concentrations of rhein and archen (20, 40 and 80 µmol/L). Morphology of apoptotic cells was observed by hematoxylin-eosin staining. Karyon apoptosis was examined by fluorescence staining of DAPI. Cell apoptosis rate was detected by flow cytometry.
RESULTS AND CONCLUSION: The outcome of hematoxylin-eosin staining and DAPI staining indicated that the effect of rhein was much stronger than that of archen on the apoptosis of mesangial cells in the rats cultured with high glucose. Comparison of the apoptosis rate also indicated that the rhein had a stronger effect on the earlier and the later stage apoptotic rate of mesangial cells than archen. Both rhein and archen with different concentrations can induce apoptosis of mesangial cells, but the effect of rhein is much stronger.

Key words: organ transplantation, kidney transplantation, kidney glomerulus, diabetic nephropathies, apoptosis

CLC Number:

R318

Guo Yu, Li Yan, Fang Hui, Yin Xiao-xing, Wei Qun-li. Rhein and archen induce the apoptosis of mesangial cells in high glucose rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(44): 7739-7744.

Figures/Tables 8

References

[1]Shlipak M. Diabetic nephropathy: preventing progression. Clin Evid (Online). 2010 Jul 8;2010.
[2]Yamagishi S, Fukami K, Ueda S, et al. Molecular mechanisms of diabetic nephropathy and its therapeutic intervention. Curr Drug Targets. 2007;8(8):952-959.
[3]陈志红.糖尿病肾病发病机制研究进展[J].承德医学院学报,2010, 27(2):184-186.
[4]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2010:22-23.
[5]魏群利,陆晓和,夏曙辉,等．消可宁对高糖诱导的肾小球系膜细胞增殖的影响[J].中国组织工程研究与临床康复,2007,11(12): 2371-2374．
[6]李红芬,郑肇巽,马品耀,等.HE染色原理和试剂配制及染色过程中的若干问题探讨[J]. 医学信息,2011,24(4):1986-1986.
[7]田玉旺,李琳,朱红艳,等.常规HE染色易出现的问题、原因及解决方法[J].诊断病理学杂志,2008,15(6):500-502.
[8]王力,杨举伦,赵稳兴,等. 改良Ehrlich苏木精染液在 HE染色中的应用[J].临床与实验病理学杂志,2011,27(8):902-903.
[9]Aschar-Sobbi R, Abramov AY, Diao C, et al. High sensitivity, quantitative measurements of polyphosphate using a new DAPI-based approach. J Fluoresc. 2008;18(5):859-866.
[10]Smith SA, Morrissey JH. Sensitive fluorescence detection of polyphosphate in polyacrylamide gels using 4',6-diamidino-2-phenylindol. Electrophoresis. 2007;28(19): 3461-3465.
[11]潘春留,闫雷,娜仁图娜拉,等.流式细胞术研究细胞凋亡的方法与技术[J].现代生物医学进展,2009,9(4):790-793.
[12]吴建勇,赵德璋.流式细胞仪检测细胞凋亡的几种方法的比较[J].重庆医科大学学报,2010,35(9):1386-1388.
[13]李超,韩金路,王玉刚,等.流式细胞仪的工作原理及应用[J].中国实用医药,2009,4(20):235-236.
[14]Chung CY, Park YL, Song YA, et al. Knockdown of RON inhibits AP-1 activity and induces apoptosis and cell cycle arrest through the modulation of Akt/FoxO signaling in human colorectal cancer cells. Dig Dis Sci. 2012;57(2):371-380.
[15]Gao Q, Qin WS, Jia ZH, et al. Rhein improves renal lesion and ameliorates dyslipidemia in db/db mice with diabetic nephropathy. Planta Med. 2010;76(1):27-33.
[16]黄娟,陈文莉,朱红.大黄酸对输尿管梗阻大鼠肾组织纤维化的保护机制[J].中国药师,2009,12(11):1529-1531.
[17]李晓红,李蒙,陶艳蓉.大黄酸及其衍生物药理作用研究新进展[J].现代药物与临床,2010,25(6):417-421.
[18]郭禹,李岩,房卉,等. 大黄酸可影响高糖培养SD大鼠肾小球系膜细胞的凋亡[J]. 中国组织工程研究,2012,16(40):7503-7507.
[19]刘冠贤,叶任高,谭志明,等. 大黄素对狼疮性肾炎成纤维细胞生物学行为的影响[J]. 中国中西医结合杂志,2000,20(3):196-198.
[20]姜春玲.系膜细胞的功能对肾小球滤过作用的影响[J].大连医科大学学报,2003,25(1):67-69.
[21]李杰,张陆勇,江振洲.大黄素的药理学研究近况[J]. 药学进展, 2005,29(12):540-543.
[22]Su YT,Chang HL,Shyue SK,et al. Emodin induces apoptosis in human lung adenocarcinoma cells through a reactive oxy-gen species-dependent mitochondrial signaling pathway.Biochem Pharmacol. 2005;70(2):229-241 .
[23]魏建波,刘琴,钟瑜,等.大黄素对大鼠肾间质纤维化干预作用的实验研究[J].浙江中西医结合杂志,2013,23(5):337-338.
[24]李冬梅,孙禄,刘巍,等.大黄素对肾纤维化大鼠肾组织中基质金属蛋白酶组织抑制物-1表达的影响[J].齐齐哈尔医学院学报, 2010, 17:2689-2691.
[25]金国华,房向东.大黄素在延缓肾脏纤维化过程中的作用[J].山东医药,2011,51(32):114-115.
[26]董华玲,马华.中药芪红合剂对肾间质纤维化模型大鼠肾组织中基质金属蛋白酶组织抑制剂-1表达的影响[J].中国医药导报, 2008,5(28):5-8.