Preparation of a thermosensitive hydrogel scaffold combined with bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.42.015

Abstract

Abstract:

BACKGROUND: Scaffolds made of chitosan and its derivatives play an important role in cell migration and axonal regeneration. Chitosan and its derivatives have good histocompatibility, which is easy to make stem cells grow on the surface, thereby having a more broad application prospect in the nerve tissue engineering.
OBJECTIVE: To fabricate a thermosensitive hydrogel scaffold using chitosan/hydroxypropyltrimethyl ammonium chloride chitosan/sodium glycerophosphate (CS/HACC/GP), which is suitable for cell growth, and then, to observe the growth and proliferation of bone marrow mesenchymal stem cells on the scaffold.
METHODS: Chitosan was modified using quaternary ammonium salt and confirmed by Fourier transform infrared spectroscopy. The chitosan and quaternary ammonium salt of chitosan was mixed at a ratio of 8:1 to successfully prepare stable CS/HACC/GP thermosensitive hydrogel scaffold. Then, the gelling was observed, and biosafety test was conducted.
RESULTS AND CONCLUSION: Fourier transform infrared spectroscopy showed the characteristic peak of quaternary ammonium groups. Cytotoxicity test showed that rat bone marrow mesenchymal stem cells cultured in hydrogel extracts had no toxicity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test showed that hydrogel extracts exerted no significant effect on the increase in body weight, and the biological safety of the scaffold was good. Under the scanning electron microscopy, bone marrow-derived mesenchymal stem cells grew and proliferated normally in the scaffold. The results confirmed that the CS/HACC/GP thermosensitive hydrogel scaffold was successfully prepared in the experiment, which is suitable for the growth and proliferation of bone marrow mesenchymal stem cells.

Key words: hydrogel, mesenchymal stem cells, bone marrow, tissue engineering, chitosan

CLC Number:

R318

Li Yi-nan, Li Guang-feng, Feng Xin-min, Huang Cheng, Xiao Hai-xiang, Yang Jian-dong . Preparation of a thermosensitive hydrogel scaffold combined with bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(42): 7435-7441.

Figures/Tables 6

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