Combination of tanshinone IIa and astragaloside induces bone marrow mesenchymal stem cells differentiating into myocardium-like cells

doi:10.3969/j.issn.2095-4344.2013.36.021

Abstract

Abstract:

BACKGROUND: Astragaloside and tanshinone IIa are the effective components of traditional Chinese medicine treatment of myocardial ischemia, and its role in bone marrow mesenchymal stem cells differentiation into myocardium-like cells remains unclear.
OBJECTIVE: To investigate the effect of tanshinone IIa and astragaloside on the differentiation of bone marrow mesenchymal stem cells into myocardium-like cells. METHODS: The maximal non-toxic concentrations of tanshinone IIa and astragaloside were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, to define the dose of the two in the induced differentiation of bone marrow mesenchymal stem cells into myocardium-like cells. The isolated and purified bone marrow mesenchymal stem cells were divided into five groups: astragaloside group, tanshinone IIa group, astragaloside + tanshinone IIa group, 5-azacitidine group, and blank control group. The expression of gap junction connexin 43 and troponin was determined with enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The expression of gap junction connexin 43 and troponin in astragaloside group, tanshinone IIa group, astragaloside + tanshinone IIa group, 5-azacitidine group was higher than that in blank control group (P < 0.01). The astragaloside + tanshinone IIa group showed a higher expression of gap junction connexin 43 and troponin than astragaloside group and tanshinone IIa group (P < 0.01). There was no significant difference in the expression of gap junction connexin 43 and troponin between astragaloside + tanshinone IIa group and 5-azacitidine group (P > 0.05). A combined use of astragaloside and tanshinone IIa can induce bone marrow mesenchymal stem cells to differentiate into myocardium-like cells, and their joint role is better than the role of a single ingredient.

Key words: stem cells, Salvia miltiorrhiza, Astragalus membranaceus, myocardial ischemia, cell

CLC Number:

R394.2

Song Xue-yun, Long Yun, Tan Chao, Zheng Jing-hui. Combination of tanshinone IIa and astragaloside induces bone marrow mesenchymal stem cells differentiating into myocardium-like cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(36): 6515-6520.

Figures/Tables 4

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