Green fluorescent protein expression in vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.36.001

Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (36): 6381-6387.doi: 10.3969/j.issn.2095-4344.2013.36.001

Green fluorescent protein expression in vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells

Yang Yang¹, Huang Zheng-de¹, Tian Xue-fei¹, Zhou De-sheng², Li Xin-hui1, Hu Hua², Yang Yan-hong¹

¹Diagnostics Institute of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Laboratory of Pathophysiology, National Key Subjects of State Administration of Traditional Chinese Medicine, Laboratory of Key Laboratory of Chinese Internal Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China; ²Department of Neurology, First Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China

Received:2012-12-07 Revised:2013-01-18 Online:2013-09-03 Published:2013-09-03
Contact: Huang Zheng-de, M.D., Professor, Doctoral supervisor, Diagnostics Institute of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Laboratory of Pathophysiology, National Key Subjects of State Administration of Traditional Chinese Medicine, Laboratory of Key Laboratory of Chinese Internal Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China Hzd112@136.com
About author:Yang Yang☆, Studying for doctorate, Physician, Diagnostics Institute of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Laboratory of Pathophysiology, National Key Subjects of State Administration of Traditional Chinese Medicine, Laboratory of Key Laboratory of Chinese Internal Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China antonyyangoo1@126.com
Supported by:
the National Natural Science Foundation of China, No. 30973750*; Hunan Provincial Innovation Foundation For Postgraduate in 2012, No. CX2012B335*

Abstract

Abstract:

BACKGROUND: Vascular endothelial growth factor is a potent angiogenesis and permeability inducible factor. Vascular endothelial growth factor 165 and vascular endothelial growth factor 121 are mainly expressed in vivo, with a strong role of angiogenesis.
OBJECTIVE: To observe the feasibility of vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells to differentiate into vascular endothelial cells.
METHODS: Bone marrow derived mesenchymal stem cells were isolated and collected from 50 g Sprague-Dawley rats,and identified by flow cytometry. The plasmid pGLV-EF1a carrying a vascular endothelial growth factor 165 gene was transfected to the mesenchymal stem cells using lentiviral. Expression of green fluorescent protein was observed under a fluorescence microscope.
RESULTS AND CONCLUSION: After 12 hours of transfection, expression of green fluorescent protein was observed, increased at 48 hours, peaked at 72 hours and gradually declined thereafter. Results prove that vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells have the expression of green fluorescent protein, indicating successful transfection. It is feasible to induce bone marrow mesenchymal stem cells to differentiate into vascular endothelial cells.

Key words: stem cells, vascular endothelial growth factors, transfection, green fluorescent proteins

CLC Number:

R394.2

Yang Yang, Huang Zheng-de, Tian Xue-fei, Zhou De-sheng, Li Xin-hui, Hu Hua, Yang Yan-hong. Green fluorescent protein expression in vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(36): 6381-6387.

Figures/Tables 3

References

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Green fluorescent protein expression in vascular endothelial growth factor 165 gene transfected bone marrow mesenchymal stem cells

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