Quantitative analysis of transplanted effect of human amniotic epithelial cells in mice with acute liver injury

doi:10.3969/j.issn.2095-4344.2013.32.014

Abstract

Abstract:

BACKGROUND: There are many preliminary studies on the survival, metaptosis, and correlation characteristics of human amniotic epithelial cells after transplanted into the animals, but there are no reports on the quantitative analysis of the transplantation effect.
OBJECTIVE: To make quantitative analysis on serum biochemical function of liver and the expression of human albumin in mice received passaged human amniotic epithelial cells transplantation in spleen.
METHODS: Forty nude mice were randomly divided into four groups (n=10 in each group): hepatectomy+cell transplantation 2 weeks group, hepatectomy+cell transplantation 4 weeks group, hepatectomy+normal saline group (treated with partial hepatectomy) and hepatectomy+cell transplantation group (transplanted with 0.2 mL passaged human amniotic epithelial cells with 5×106 under spleen, and the blood were collected at 2 and 4 weeks after transplantation). The mice in the hepatectomy+normal saline group were treated with splenic injection of 0.2 mL normal saline; the cell transplantation group did not receive hepatectomy, and transplanted with 0.2 mL passaged human amniotic epithelial cells with 5×106 under spleen. The histological and morphological changes of the liver and spleen in each group as well as the expressions of serum alanine aminotransferase, aspartate aminotransferase and human serum albumin in each group were detected, and the quantitative analysis of human serum albumin expression was performed.
RESULTS AND CONCLUSION: There was no obvious morphological change after human amniotic epithelial cells transplanted into the acute liver injury mice for 4 weeks, but specific cells could be detected by histological method. The serum levels of alanine aminotransferase, aspartate aminotransferase and human serum albumin were improved obviously, and the human albumin could be detected in serum, the level of human albumin at 4 weeks after transplantation was significantly increased than 2 weeks after transplantation. Human amniotic epithelial cells can survive for more than 4 weeks after transplanted into the liver injury mice, and can still express partial characteristics and functions of hepatocyte-like cells, improve the liver function, thus treating acute liver injury.

Key words: stem cells, stem cell transplantation, human amniotic epithelial cells, intrasplenic transplantation, acute liver injury, liver function, human albumin, alanine aminotransferase, aspartate aminotransferase, quantitative analysis, liver, spleen, stem cell photographs-containing paper

CLC Number:

R394.2

Luo Hong-wu, Xun Quan, Huang Xiang-jun, Huang Fei-zhou. Quantitative analysis of transplanted effect of human amniotic epithelial cells in mice with acute liver injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5834-5839.

Figures/Tables 5

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