Effect of bone marrow mononuclear cell transplantation on angiogenesis and expression of cytokines following myocardial infarction

doi:10.3969/j.issn.2095-4344.2013.32.015

Abstract

Abstract:

BACKGROUND: Cell transplantation offers a new promise of rebuilding the damaged myocardium. But the results of them are not consistent. It is not clear if the transplanted cells can permanently improve heart function and the mechanism underlying this therapeutic effect.
OBJECTIVE: To study the effect of intracoronary autologous bone marrow mononuclear cell transplantation on cardiac function, and angiogenesis and cytokine production in canines with acute myocardial infarction.
METHODS: Left anterior descending coronary artery ligation was used to produce acute myocardial infarction models in hybrid canines. Bone marrow mononuclear cells were harvested by using puncture of anterior crest and posterior superior iliac spine to prepare cell suspension. Sixteen hybrid canines were randomly divided into transplantation group (n=10) and control group (n=6). Bone marrow mononuclear cells (transplantation group, n=10) or normal saline (control group, n=6) were intracoronarily infused into infarction-related arteries 2 hours after acute myocardial infarction. To evaluate the heart function, we used echocardiography at 2 hours and 6 weeks after acute myocardial infarction. Capillary density was assessed 6 weeks after transplantation by using von Willebrand factor test. The mRNA levels of vascular endothelial growth factor 188, vascular endothelial growth factor 164, basic fibroblast growth factor and matrix metalloproteinase-9 in the infarct area were determined by reverse transcription-PCR at 6 weeks after transplantation.
RESULTS AND CONCLUSION: In contrast to the control group, ejection fraction and stroke volume at 6 weeks after transplantation increased significantly in the transplantation group. The transplantation group had a greater amount of new vessels in the peri-infarct area than the control group. Compared with the control group, the mRNA levels of vascular endothelial growth factor 188, vascular endothelial growth factor 164, and basic fibroblast growth factor significantly increased in the transplantation group, but the mRNA level of matrix metalloproteinase-9 significantly decreased in the transplantation group. These findings suggest that intracoronary transplantation of autologous bone marrow mononuclear cells may improve the cardiac function, and increase capillary density, especially in the border zone of infarcted myocardium. Otherwise, bone marrow mononuclear cell transplantation can increase the mRNA levels of vascular endothelial growth factor 188, vascular endothelial growth factor 164, and basic fibroblast growth factor, but decrease the mRNA level of matrix metalloproteinase-9.

Key words: stem cells, stem cell transplantation, bone marrow mononuclear cell, intracoronary infusion, cardiac function, capillary density, matrix metalloproteinase-9, cytokine, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Tang Jie, Chen Tao, Mi Jie, Xu Ai-guo, Wang Yong-de, Zhang Jian, Qi Xiang-qian. Effect of bone marrow mononuclear cell transplantation on angiogenesis and expression of cytokines following myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5840-5846.

Figures/Tables 3

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