Effects of low-frequency electromagnetic fields on skin wound healing after the transplantation of gene modified epidermal stem cells

doi:10.3969/j.issn.2095-4344.2013.32.013

Abstract

Abstract:

BACKGROUND: The repair and management of full-thickness skin defects resulting from burns and chronic wounds remain a significant unmet clinical challenge. Using epidermal stem cells and keratinocyte growth factor for full-thickness wound repair is a promising approach. Low-frequency electromagnetic fields which are a non-invasive physical stimulation therapy have been recognized as a good method to enhance wound healing.
OBJECTIVE: To develop a new strategy to accelerate wound healing by transplanting transfected epidermal stem cells and keratinocyte growth factor and treating with low-frequency electromagnetic fields in a mouse model.
METHODS: Epidermal stem cells from Sprague-Dawley neonatal rats were isolated and cultured in vitro, then the cells were labeled with 5-bromo-2-deoxyuridine and transfected by Ad-KGF, a recombinant adenovirus carrying the keratinocyte growth factor. Mice were given to create full thickness skin wound on the dorsum and randomly assigned to four groups: control group, transplantation of epidermal stem cells group, transplantation of keratinocyte growth factor gene modified epidermal stem cells group, and transplantation of keratinocyte growth factor gene modified epidermal stem cells plus low-frequency electromagnetic field exposure group.
RESULTS AND CONCLUSION: The best healing pattern was observed in the keratinocyte growth factor gene modified epidermal stem cells plus low-frequency electromagnetic field exposure group (P < 0.05) at days 9 and 16. 5-Bromo-2-deoxyuridine labeled cells existed in the wound in the treated groups at day 9. A significantly increased expression of endogenous keratinocyte growth factor was detected in the transplantation of Keratinocyte Growth Factor gene modified epidermal stem cells group, and transplantation of keratinocyte growth factor gene modified epidermal stem cells plus low-frequency electromagnetic field exposure group at day 16. A well-advanced epithelialization was observed in transplantation of keratinocyte growth factor gene modified epidermal stem cells plus low-frequency electromagnetic field exposure group at days 16 and 30. These results suggest that low-frequency electromagnetic fields enhanced wound healing following the transplantation of keratinocyte growth factor gene modified epidermal stem cells.

Key words: stem cells, stem cell transplantation, low-frequency electromagnetic field, epidermal stem cells, keratinocyte growth factor, full-thickness skin wound, wound healing, provincial grants-supported paper, stem cell photographs- containing paper

CLC Number:

R394.2

Liang Ling, Li Xin-ping, Bai Wen-fang, Bai Li-ming, Zhu Hong-xiang, Xu Wei-cheng, Feng Yu, Wang Xin, Chen Yi, Zhang Ming-sheng. Effects of low-frequency electromagnetic fields on skin wound healing after the transplantation of gene modified epidermal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5827-5833.

Figures/Tables 5

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