Oxidative stress effect on viability of umbilical cord-derived mesenchymal stem cells in storage solution of transplantation

doi:10.3969/j.issn.2095-4344.2013.32.007

Abstract

Abstract:

BACKGROUND: The viability of human umbilical cord-derived mesenchymal stem cells is often declined with the commonly used transplantation storage solution in clinics, which may influence the therapeutic effects of cellular transplantation. However, reasons for this are still unknown.
OBJECTIVE: To investigate the role of oxidative stress in the reduction of human umbilical cord-derived mesenchymal stem cells viability in the storage process during clinical transplantation and to observe the effects of radical scavenger on the results.
METHODS: Human umbilical cord-derived mesenchymal stem cells were harvested and cultured in normal saline for 0, 2, 4 and 6 hours at room temperature. Intracellular reactive oxygen levels were detected at those time points. Antioxidant enzyme activities and levels of malondialdehyde were measured to determine the intracellular oxidative stress levels after storage. Cell adhesion rate changes were retested after adding N-acetyl cysteine to the storage solution.
RESULTS AND CONCLUSION: The reactive oxygen levels in human umbilical cord-derived mesenchymal stem cells were increased significantly after normal saline storage and levels of malondialdehyde were increased in a time-dependent manner. Activities of superoxide dismutase, catalase and glutathione peroxidase were all reduced. Addition of N-acetyl cysteine into the storage medium decreased the reactive oxygen levels and improved the human umbilical cord-derived mesenchymal stem cells viabilities. Experimental findings indicate that, increased reactive oxygen species in human umbilical cord-derived mesenchymal stem cells is one of the reasons for reduced cell viability. Adding the radical scavenger N-acetyl cysteine can improve the storage effects of human umbilical cord-derived mesenchymal stem cells.

Key words: stem cells, umbilical cord/umbilical blood stem cells, human umbilical cord-derived mesenchymal stem cells, oxidative stress, active oxygen, senescence, glutathione, malondialdehyde, superoxide dismutase, stress level, stem cell photographs-containing paper

CLC Number:

R394.2

Niu Yu-hu, Chen Yan, Zhang Jian-lin, Lei Xin, Dong Yan-ting, Cui Lei, Niu Bo . Oxidative stress effect on viability of umbilical cord-derived mesenchymal stem cells in storage solution of transplantation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5785-5792.

Figures/Tables 9

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