Autologous bone marrow mesenchymal stem cells and related chemokines in
fracture microenvironment

doi:10.3969/j.issn.2095-4344.2013.27.019

Abstract

Abstract:

BACKGROUND: The oriented migration of bone marrow mesenchymal stem cells may depend on the interaction between local chemotactic factors and cell surface receptors. However, which chemotactic factors may mediate the oriented migration of bone marrow mesenchymal stem cells towards the fracture site remains unclear.
OBJECTIVE: To tag autologous bone marrow mesenchymal stem cells, evaluate its role in bone healing, and detect the highly expressed factors associated with migration of bone marrow mesenchymal stem cells in the microenvironment.
METHODS: The fluorescence/chimeric C57BL/6 mouse models were established, then left shankbone fracture models were also produced. The percentages of green fluorescent protein positive cells to all cells at the fracture site and the percentage of osteoblasts differentiated from bone marrow mesenchymal stem cells to all the osteoblasts were detected at different time points. The role of bone marrow mesenchymal stem cells in the fracture repairing was evaluated. The levels of chemotactic factors protein expression at the fracture site in different time points were detected with immunohistochemistry technology.
RESULTS AND CONCLUSION: The percentage of green fluorescent protein positive cells to all cells at the fracture site was (3.011±0.911)%, (9.031±0.145)%, (12.064±0.145)% at 1, 5, 14 days postoperatively; and osteoblasts differentiated from bone marrow mesenchymal stem cells accounted for 50% of all the osteoblasts. After fracture, the stromal cell derived factor-1, colony stimulating factor, hepatocyte growth factor, monocyte chemoattractant protein-1, and matrix metalloproteinases-9 were expressed to varying degrees in the microenvironment, while the expression of granulocyte colony-stimulating factor was negative. The expression of stromal cell derived factor-1 in the fracture microenvironment was the highest, mainly due to the migration of bone marrow mesenchymal stem cells. Experimental findings indicate that, autologous bone marrow mesenchymal stem cells participate in and play an important role in bone healing. The stromal cell derived factor-1 plays an important role in promoting bone marrow mesenchymal stem cells migration and promoting bone healing.

Key words: stem cells, bone marrow-derived stem cells, green fluorescence protein, bone marrow mesenchymal stem cells, chimera, fracture, chemokine, stromal cell derived factor-1, colony stimulating factor, hepatocyte growth factor, monocyte chemoattractant protein-1, matrix metalloproteinases-9, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Zhang Xian-yu, Wang Wei, Chen Wei, Chen Jian-mei, Xu Hao . Autologous bone marrow mesenchymal stem cells and related chemokines in
fracture microenvironment[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.019.

Figures/Tables 9

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