Myogenetic potential of rabbit adipose-derived stromal cells

doi:10.3969/j.issn.2095-4344.2013.27.011

Abstract

Abstract:

BACKGROUND: Myogenetic seed cells are the fundamental for construction of tissue-engineered smooth muscle, and optimizing the amplification of seed cells is the key in further clinical application.
OBJECTIVE: To analyze the myogenetic potential of rabbit adipose-derived stromal cells induced with the combination of MyoD, transforming growth factor β1 and 5-azacytidine, and to investigate a new way to induce cells.
METHODS: The rabbit abdominal fats were isolated and then the adipose-derived stromal cells were separated by collagenase digestion method for myogenetic induction with different methods: 5-azacytidine induction group, MyoD+transforming growth factor β1 induction group, 5-azacytidine+MyoD+transforming growth factor β1 combination induction group. The blank control group was set. The morphological characteristics of cells were observed at 1, 4, 8, 12, 16, 20, 24 and 28 days after induction, and the collagen type Ⅲ level were detected with 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and immunohistochemistry.
RESULTS AND CONCLUSION: Compared with the other groups, the cell activity was higher in the combination induction group and reached peak at 16 days after induction, the number and volume of myotubes were increased at 20 days with regular arrangement, and desmin showed strong expression. Cell cycle showed the ratio of adipose-derived stromal cells in the DNA replication phase was increased in the combination induction group, the ratio of cells in the clearance period was decreased; the level of collagen type Ⅲ was increased significantly, and the difference was significant. The results indicate that 5-azacytidine combined with multiple factors can promote the differentiation of adipose-derived stromal cells into myoblasts with significant cell proliferation, which is considered as the ideal method to in vitro induction of myoblasts.

Key words: stem cells, adipose-derived stem cells, adipose-derived stromal cells, transforming growth factor β1, myogenetic differentiation factor, 5-azacytidine, collagen type Ⅲ, myoblastic induction, myoblasts, cell culture, desmin, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Liu Jing, Lin Wan-jun, Liu Yin-Zhong, Li Xiao-mian, Guo Hong-gang. Myogenetic potential of rabbit adipose-derived stromal cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.011.

Figures/Tables 8

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