Chinese Journal of Tissue Engineering Research
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Zhou Ting-ting1, Wei Chao1, Chen Xiao-dong2, Li Hai1, Wang Zheng1
Received:
2012-12-10
Revised:
2013-01-24
Online:
2013-07-02
Published:
2013-07-02
Contact:
Li Hai, M.D., Associate professor, Department of Digestion, Renji Hospital of Shanghai Jiao Tong University Medical School, Shanghai 200001, China
haili_17@yahoo.com
Corresponding author: Wang Zheng, M.D., Associate professor, Department of Digestion, Renji Hospital of Shanghai Jiao Tong University Medical School, Shanghai 200001, China
zheng.w.dr@gmail.com
About author:
Zhou Ting-ting★, Studying for master’s degree, Department of Digestion, Renji Hospital of Shanghai Jiao Tong University Medical School, Shanghai 200001, China
Guxinglei1005@126.com
Supported by:
the Specific Foundation for Key Laboratory of Digestion Medicine, Ministry of Health, No. 30972751*; the National Natural Science Foundation of China, No. 30971468
CLC Number:
Zhou Ting-ting, Wei Chao, Chen Xiao-dong, Li Hai, Wang Zheng. Primary culture of umbilical cord-derived mesenchymal stem cells in serum free medium[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.006.
2.3 不同培养体系经典干细胞原代表面标志的变化 两种培养体系下获得的细胞均高表达CD44[37],CD73,CD166[38-39],CD105和HLA-ABC(约为95%)等,不表达CD34,CD31,CD80,HLA-DR,CD45(<2%)等[40],见图3。 2.4 不同培养体系下干细胞原代亚群表面标志的变化 两种培养体系下干细胞亚群标志SSEA-4表达很低,在含血清培养体系中CD146先升高后下降[41],最高超过50%,SSEA-4表达量最高值不超过7%[42-43]。无血清培养条件下,以上表面标志均没有明显变化。另外,两种培养条件下均不表达骨髓间充质干细胞高表达的CD271[44],见图4。 2.5 不同培养条件下成软骨分化结果 含血清培养条件下和无血清培养条件下,脐带间充质干细胞均可以成软骨分化成功,见图5。 2.6 不同培养条件下集落形成实验检测结果的变化 无血清培养体系相对于含血清体系,原代干细胞增殖能力明显提高,并且在培养到14 d时达到高峰;含血清培养体系中,随着培养时间的延长,形成的集落数逐渐呈下降趋势。另外,在不同的培养时间,观察发现集落形成实验中无血清培养体系下形成的集落数均多于含血清培养体系下形成的集落数,并且在11 d和14 d时,差异有显著性意义,见图6。"
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