Chinese Journal of Tissue Engineering Research
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Wang Feng, Fu Zhi-hou
Received:
2013-03-15
Revised:
2013-04-18
Online:
2013-07-02
Published:
2013-07-02
Contact:
Fu Zhi-hou, M.D., Chief physician, Professor, Master’s supervisor, Postgraduate Training Unit of Liaoning Medical University, General Hospital of Jinan Military Region, Jinan 250031, Shandong Province, China
jzgbfzh@yahoo.com.cn
About author:
Wang Feng★ Studying for master’s degree, Postgraduate Training Unit of Liaoning Medical University, General Hospital of Jinan Military Region, Jinan 250031, Shandong Province, China
medouni@163.com
Supported by:
the Postgraduate Foundation of Liaoning Medical University
CLC Number:
Wang Feng, Fu Zhi-hou. Bone marrow mesenchymal stem cells combined with allogeneic bone for cancellous bone defects[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.004.
2.1 实验动物数量分析 纳入兔36只,其中2只兔因造模失败,后期1只兔子因感染死亡,1只取材失败,均直接剔除,未补充数量,最终32只兔计入结果分析。 2.2 骨髓间充质干细胞复合异体骨移植后松质骨缺损兔一般形态 植入后第1天,兔活动量与进食均较少。植入后第2天,兔均可恢复正常习性,饮食活动正常。兔切口初期略有红肿,稍有渗出,但后期切口均愈合良好。 股骨外髁表面观察:模型组与对照组股骨髁骨缺损处随时间变化,骨质形成均比较明显,逐步恢复平坦外观,植入后12周骨皮质均已修复。 股骨髁剖面观察:模型组:植入后4周,植入骨块之间明显粘连,但骨块之间较易分离,与周围骨质分界清晰;植入后8周,植入骨块间连接紧密,不易分离,与周围骨质界限变模糊;植入后12周,难以区分植入骨块与周围骨质,骨缺损基本修复。 对照组:植入后4周,植入骨块之间有少量粘连,植入骨块、骨床之间基本呈游离状态;植入后8 周,植入骨块明显粘连,但较容易剥离,与周围骨质分界清晰;植入后12周,植入骨块间级与周围骨质彼此粘连,分离困难,不易区分周围骨质与植入骨块。 2.3 骨髓间充质干细胞复合异体骨移植后松质骨缺损兔X射线检查结果 植入后4周,模型组骨缺损处密度高低不均,缺损区边缘模糊;对照组骨缺损处可见大量植入骨块高密度影,缺损区边缘清晰。植入后8周,模型组骨缺损处可见大量密度与周围骨质一致区域,缺损区域缩小,对照组骨缺损处植入骨块高密度影之外可见部分区域与周围骨质密度一致,缺损区域明显。植入后12周,模型组骨缺损处仍可见植入骨块高密度影,但大多区域基本与周围骨质一致,难以区分原缺损区域;对照组骨缺损处亦可见植入骨块高密度影,大量与周围骨质密度一致区域,但原缺损区域仍模糊可见。 2.4 骨髓间充质干细胞复合异体骨移植后松质骨缺损兔股骨髁生物力学指标变化 植入后12周,与对照组相比,模型组股骨髁最大压力载荷、载荷/应变比值较高确 (P < 0.05),最大应变位移较低(P < 0.05)。见表1。"
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