Silk/poly(lactic-co-glycolic acid) scaffold degradation fluid and proliferation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.25.016

Abstract

Abstract:

BACKGROUND: Previous studies showed that silk/poly(lactic/co-glycolic acid) copolymer scaffold has good cytocompatibility and no cytotoxicity.
OBJECTIVE: To observe the effects of degradation fluid of silk/poly(lactic-co-glycolic acid) braided scaffold on the proliferation and growth of rabbit bone marrow-derived mesenchymal stem cells in vitro.
METHODS: The silk/poly(lactic-co-glycolic acid) braided scaffold was degraded in complete culture medium for 14 weeks in vitro, and medium was replenished every week. The pH value of degradation fluid of the scaffold was determined every week. Rabbit bone marrow mesenchymal stem cells were cultured with degradation fluid and complete medium each 100 µL (experimental group), while negative control group was added with complete medium 200 µL, culturing for 4 days. The proliferation and growth of mesenchymal stem cells was detected with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
RESULTS AND CONCLUSION: (1) pH value changes of degradation fluid of silk/poly(lactic-co-glycolic acid) scaffold: pH value declined slowly with the first 3 weeks, from 7.00 to 6.89. However, the value went down quickly at 4 weeks, maintained a low level at 6-11 weeks, between 5.16 and 5.67. Then pH values increased at 12-14 weeks, rising to 6.95. (2) Morphology of mesenchymal stem cells: The proliferation and growth of mesenchymal stem cells in experimental group and negative control group were basically similar. Degradation fluid inhibited the cell growth at degradation 7-10 weeks, exhibiting relatively less counts and sparse arrangement of the cells, while degradation fluid in other weeks had no obviously inhibition on the cell growth. (3) Proliferation of mesenchymal stem cells: Degradation fluid of the scaffolds at 1-6 weeks and 11-14 weeks had no significant impact on cell proliferation, and the relative growth rates of the cells were all over 92.1%, with toxicity grade 0 or 1 level; degradation fluid at 7-10 weeks had an inhibition effect on cell proliferation, but relative growth rates were 82.5%-87.9%, with toxicity grade 1 level, as qualified. Experimental findings indicate that, the degradation fluid of silk/poly(lactic-co-glycolic acid) braided scaffold has a good cytocompatibility.

Key words: biomaterials, material biocompatibility, silkworm silk, poly(lactic-co-glycolic acid), degradation fluid, bone marrow mesenchymal stem cells, proliferation, pH value, MTT assay, National Natural Science Foundation of China

CLC Number:

R318

Zhang Wen-yuan, Yang Ya-dong, Li Ying, Zhang Ke-ji, Fang Guo-jian, Tang Liang, Li Yue-zhong, Wang Han, Lu Ming-yang. Silk/poly(lactic-co-glycolic acid) scaffold degradation fluid and proliferation of bone marrow mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(25): 4676-4683.

Figures/Tables 6

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