Preparation and selection of a new sodium alginate-heparin composite coating with multi-aldehyde groups

doi:10.3969/j.issn.2095-4344.2013.25.015

Abstract

Abstract:

BACKGROUND: Preliminary experiments have demonstrated that composite coating of sodium alginate and heparin with multi-aldehyde groups has excellent biocompatibility and hemocompatibility. However, little is know about the optimal condition and pattern of new coating preparation.
OBJECTIVE: To prepare a new composite coating of sodium alginate and heparin, and to optimize the coating preparation conditions.
METHODS: The optimal coating conditions of sodium alginate (or heparin) were screened by orthogonal experiment, to obtain three coating patterns of sodium alginate/heparin composite. Composite coating was covalently bonded on the polyvinyl chloride surface which was preprocessed by amination/chemical modification. The screening methods of optimal composite coating pattern included coating quantification, protein adhesion test and surface contact angle. Anticoagulation properties of optimum composite coating model were assessed.
RESULTS AND CONCLUSION: The optimum coating condition of oxidated sodium alginate in composite coating was 50% concentrated sulphuric acid, 0.05% polyethyleneimine, pH 3.5, reaction temperature 40℃ and 2 g/L oxidated sodium alginate. The optimum coating condition of heparin in composite coating was 70% concentrated sulphuric acid, 0.1% polyethyleneimine, pH 3.5, reaction temperature 30 ℃ and 0.1 g/L low molecular nitrous acid-converted heparin. The quantities of oxidated sodium alginate and heparin in optimum composite coating pattern were (4.07±1.35) and (2.13±0.24) μg/cm². And the composite coating possesses fine anticoagulant property and biocompatibility.

Key words: biomaterials, tissue-engineered composite scaffolds, sodium alginate, heparin, composite coating, covalent bond, oxidation, diazotization, biocompatibility, anticoagulant property, provincial grants-supported paper

CLC Number:

R318

Gao Wen-qing, Li Tong, Yu Mei-li, Hu Xiao-min, Duan Da-wei, Xu Jin. Preparation and selection of a new sodium alginate-heparin composite coating with multi-aldehyde groups [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(25): 4668-4675.

Figures/Tables 4

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