Combination of cryopreserved hydroxyapatite/bone marrow mesenchymal stem cells repairs rabbit radial defects

doi:10.3969/j.issn.2095-4344.2013.25.010

Abstract

Abstract:

BACKGROUND: Previous studies have showed that hydroxyapatite combined with bone marrow mesenchymal stem cells has a good effect on bone defects, but the repair effect of cryopreserved composite material is unclear.
OBJECTIVE: To observe the effect of cryopreserved hydroxyapatite/bone marrow mesenchymal stem cells on bone defects.
METHODS: Twenty-seven Japanese rabbits were used to prepare 10 mm radial defect models and then randomly divided into cryopreserved composite group, fresh composite group and hydroxyapatite group. The three groups were respectively implanted with cryopreserved hydroxyapatite/bone marrow mesenchymal stem cells which were cryopreserved at -80℃ for 3 months, fresh hydroxyapatite/bone marrow mesenchymal stem cells and pure hydroxyapatite. After 8 and 12 weeks, general observation, X-ray observation and hematoxylin-eosin staining were performed, and biomechanical test was done at 12 weeks.
RESULTS AND CONCLUSION: At postoperative 12 weeks, bone defects were almost healed in the cryopreserved composite group and fresh composite group. Mature trabecular bone was seen, some canals were of patency, and good shape was found. While in the hydroxyapatite group, callus formation was less, only part of bone defects were healed, shaping was poor, and new bone formation was also less than the other two groups (P < 0.05). In addition, the maximal loading in the cryopreserved composite group and fresh composite group was significantly higher than that in the hydroxyapatite group (P < 0.05). These findings indicate that the cryopreserved hydroxyapatite/bone marrow mesenchymal stem cells composite material is similar to the fresh one in the repair capacity.

Key words: biomaterials, bone tissue construction, hydroxyapatite, bone marrow mesenchymal stem cells, composite scaffold materials, cryopreservation, radial defects, provincial grants-supported paper

CLC Number:

R318

Xing Zhi-yuan, Zhang Ji-bo, Kong Ling-ju, Liu Jian-sheng, Zheng De-yu. Combination of cryopreserved hydroxyapatite/bone marrow mesenchymal stem cells repairs rabbit radial defects[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(25): 4629-4636.

Figures/Tables 5

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