Danhong injection plus bone marrow mesenchymal stem cell transplantation fortreatment of cerebral infarction in rats

doi:10.3969/j.issn.2095-4344.2013.23.015

Abstract

Abstract:

BACKGROUND: Simple bone marrow mesenchymal stem cell transplantation in repair of damaged brain tissues does not exhibit ideal effect.
OBJECTIVE: To investigate the effect of Danhong injection combined with bone marrow mesenchymal stem cell transplantation on the treatment of cerebral infarction in rats.
METHODS: The models of middle cerebral artery occlusion were established by suture method and randomly divided into three groups. Model group received tail vein injection of PBS. Danhong injection group received tail vein injection of 2 mL/kg Danhong injection. Combination group received injection of
2 mL/kg Danhong injection + 2.0×10⁹/L bone marrow mesenchymal stem cell suspension, for 5 consecutive days, once a day.
RESULTS AND CONCLUSION: At 2 weeks following bone marrow mesenchymal stem cell transplantation, the neurological function scores in the combination group were significantly better than those in the model group and Danhong injection group (P < 0.05). Cerebral infarct volume in the combination group was significantly less than that in the model and Danhong injection groups at 3 weeks following transplantation (P < 0.05). Histopathological observation showed that the reduced degree of tissue injury was larger in the combination group than the Danhong injection and model groups. Results indicated that Danhong injection combined with bone marrow mesenchymal stem cell transplantation on the treatment of rats with cerebral infarction showed remarkable effects and protected brain cells.

Key words: stem cells, stem cell transplantation, bone marrow mesenchymal stem cells, Danhong injection, transplantation, rats, cerebral infarction, tail vein, injection, stem cell photographs-containing paper

CLC Number:

R394.2

Zhang Peng, Zhou Guo-qing, Sun Jing-jing. Danhong injection plus bone marrow mesenchymal stem cell transplantation fortreatment of cerebral infarction in rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(23): 4286-4291.

Figures/Tables 5

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