Effects of artificial dura on inflammation factor concentrations in blood and drainage fluid after lumbar surgery

doi:10.3969/j.issn.2095-4344.2013.21.022

Abstract

Abstract:

BACKGROUND: Research has shown that artificial dura applied for prevention and reduction of lumbar postoperative scarring has achieved good results, but whether it can reduce the releasing of inflammatory cytokines after lumbar surgery is not clear at present.
OBJECTIVE: To explore the effects of artificial dura on concentration of inflammation factors in blood and drainage fluid after lumbar surgery.
METHODS: A total of 40 patients with prolapse of lumbar intervertebral disc and lumbar spinal stenosis in the Institute of Orthopedics and Traumatology, General Hospital of Beijing Military Area Command of Chinese PLA between March and July 2012, who underwent posterior lumbar decompression, intervertebral disk removal and intervertebral fusion internal fixation, were selected. They were randomly assigned to two groups. Patients using the artificial dura were considered as the experimental group, while the others were considered as the control group. In addition, an additional 10 healthy volunteers were tested as the blank control group. The concentrations of interleukin-1β, interleukin-6 and tumor necrosis factor-α in the blood samples were determined before surgery, at 2 and 3 days following surgery in the three groups. Enzyme-linked immunosorbent assay was used to measure mass concentrations of interleukin-1β, interleukin-6 and tumor necrosis factor-α in drainage fluid at 1, 2 and 3 days after surgery in the experimental and control groups.
RESULTS AND CONCLUSION: (1) Drainage fluid: the concentration of interleukin-1β, interleukin-6 and tumor necrosis factor-α at different time points in experimental group was significantly lower than that in the control group (P < 0.05), and there were no fever, allergies or foreign body rejection in the experimental group. Patients in experimental group had a less oozing compared with the control group (P < 0.05). (2) Blood: the concentrations of interleukin-1β and tumor necrosis factor-α were significantly lower in the experimental group than those in the control group at 3 days (P < 0.05). The concentrations of interleukin-1β, interleukin-6 and tumor necrosis factor-α in the experimental and control groups were significantly higher than those in the blank control group at various time points (P < 0.05). These results suggested that artificial dura can reduce the concentrations of interleukin-1β, interleukin-6 and tumor necrosis factor-α after lumbar surgery in fluid drainage, and slightly decrease the concentrations of inflammatory factors in peripheral blood.

Key words: biomaterials, basic experiments of biomaterials, membrane biomaterials, lumbar postoperation, artificial dura, inflammation factor, drainage fluid, interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha

CLC Number:

R318

Yin Yan-bin, Li Fang, Zhao Guang-min, Zhang Chang-meng. Effects of artificial dura on inflammation factor concentrations in blood and drainage fluid after lumbar surgery[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.022.

Figures/Tables 4

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