Copper-taurine regulates vascular endothelial growth factor A expression during infected wound healing process in rats

doi:10.3969/j.issn.2095-4344.2013.21.021

Abstract

Abstract:

BACKGROUND: Copper has a broad spectrum bactericidal characterization and plays an important role in wound healing. Authors’ previous study has obtained copper-taurine organic compound which provides a better bioavailability to the human body.
OBJECTIVE: To detect the antibacterial activity of copper-taurine and study its effect on vascular endothelial growth factor A expression during infected wound healing.
METHODS: (1) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to examine the minimum inhibitory concentration of copper-taurine against Staphylococcus aureus. (2) Seventy-two Sprague-Dawley rats were randomly divided into copper-taurine and normal saline groups. A 1.5-cm Staphylococcus aureus infected wound was made on the back of rats. Copper-taurine group was topically treated with copper-taurine liquor 1 mL per wound, and normal saline group treated with normal saline 1 mL per wound.
RESULTS AND CONCLUSION: (1) The minimum inhibitory concentration of the copper-taurine against Staphylococcus aureus was 4 g/L. (2) On days 7 and 10, the percent wound contraction of the copper-taurine group was higher than that of the normal saline group (P < 0.05). Wounds in two groups healed until day 21. (3) Histological observation: On days 7 and 10, in the copper-taurine group, the granulation was matured showing capillary vertically oriented, robust fusiform fibrocytes and moderate well-arranged collagen. (4) Immunohistochemical examination of vascular endothelial growth factor A expression: On day 3, the expression of vascular endothelial growth factor A was significantly higher in the copper-taurine group compared with the normal saline group (P < 0.01). On days 7 and 10, the copper-taurine group still expressed higher vascular endothelial growth factor A than the normal saline group (P < 0.05). There was no difference at other time points (P > 0.05). In conclusion, topically applied copper-taurine on rat infected cutaneous wound can reduce the quantity of microbe, improve vascular endothelial growth factor A expression during wound healing, thus improving the quality of wound healing.
Key Words: biomaterials; basic experiments of biomaterials; taurine; copper; antibacterial; wound healing; vascular endothelial growth factor A; Staphylococcus aureus; infection; endothelial cells; fibroblasts; scar; National Natural Science Foundation of China

Key words: biomaterials, basic experiments of biomaterials, taurine, copper, antibacterial, wound healing, vascular endothelial growth factor A, Staphylococcus aureus, infection, endothelial cells, fibroblasts, scar, National Natural Science Foundation of China

CLC Number:

R318

Wang Shou-yu, Tian Xi-liang, Lü De-cheng. Copper-taurine regulates vascular endothelial growth factor A expression during infected wound healing process in rats[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.021.

Figures/Tables 10

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