Abstract:

BACKGROUND: Studies have shown that silver-zirconium phosphate in the six kinds of well-known brands of oral nanosilver inorganic antibacterial agents has the best sterilization and safety.  
OBJECTIVE: To explore the influence of silver-zirconium phosphate antimicrobial agent FUMAT T200-4 on the cell growth, morphology, ultrastructure, insulin-like growth factor 1 expression and secretion of 293T cells in vitro.
METHODS: FUMAT T200-4 was prepared into diluents of 5, 2.5, 1.25, 0.625 g/L mass concentration. Cell Counting Kit-8 was employed to detect the impact on 293T cells proliferation with diluents of variousc oncentrations. After 293T cells were treated with1.25 g/L FUMAT T200-4, the cell morphology was observed using inverted phase contrast microscope. And further transmission electron microscope was used to observe the ultrastructure of 293T cells. In addition, reverse transcription-PCR was used to investigate cellular insulin-like growth factor mRNA expression and enzyme linked immunosorbent assay was used to detect the content of insulin-like growth factor protein in the supernatant solution.  
RESULTS AND CONCLUSION: After 293T cells were treated with FUMAT T200-4 for 48 hours, the cytotoxicity of 293T cells was graded as II for of 1.25 g/L showing mild cytotoxicity and I for the other concentrations showing no toxicity. The images of inverted phase contrast microscope showed that the cell shape after treated with    1.25 g/L for 24, 36, and 48 hours became abnormal which showed mild cell toxicity, especially after 48 hours. The ultrastructure images of transmission electron microscope showed nanoparticles entered 293T cells and damaged cell membrane. There was no significant deviation in cellular insulin-like growth factor 1 mRNA and protein expression after treated with 1.25 g/L for 48 hours in contrast to the control group (P > 0.05). Silver-zirconium phosphate can influence 293T cells proliferation and growth, exhibiting certain cytotoxicity to 293T cells, and its application in dental materials still needs further study.

Key words: biomaterials, tissue-engineered oral materials, inorganic antibacterial agents, zirconium phosphate, nano, oral biomaterials, cell proliferation, cytotoxicity, biocompatibility, 293T cells, provincial grants-supported paper