Magnesium alloy modified by micro-arc oxidation can influence tumor necrosis factor-alpha and interleukin-6 production in macrophages

doi:10.3969/j.issn.2095-4344.2013.21.011

Abstract

Abstract:

BACKGROUND: Micro-arc oxidation treatment can improve the corrosion resistance of the magnesium alloy and delay the degradation rate.
OBJECTIVE: To investigate the effects of magnesium alloy with micro-arc oxidation treatment on tumor necrosis factor-alpha and interleukin-6 expression in mouse macrophage RAW264.7 cells.
METHODS: Extracts of magnesium alloy, magnesium alloy with micro-arc oxidation treatment or RPMI-1640 medium were added to RAW24.7 cells at 1 hour prior to lipopolysaccharide treatment for 24 hours. Then culture supernatants were collected for measuring the concentrations of tumor necrosis factor-alpha and interleukin-6, and cell proliferation were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.
RESULTS AND CONCLUSION: Lipopolysaccharide could significantly increase RAW264.7 cell viability. However, there was no significant difference in lipopolysaccharide-induced RAW264.7 cell viability among the three groups (P > 0.05). But the cell viability in the blank control group was significantly increased after lipopolysaccharide treatment (P < 0.01). The highest viability of RAW264.7 cells without lipopolysaccharide stimulation was determined by magnesium alloy treatment, followed by magnesium alloy modified by micro-arc oxidation treatment and RPMI-1640 medium treatment (P < 0.05). Interleukin-6 level of lipopolysaccharide-induced RAW264.7 cells with magnesium alloy treatment appeared to be increased compared to the other two groups (P < 0.05). Unlike interleukin-6 response in lipopolysaccharide-induced RAW264.7 cells, there was no significant difference in tumor necrosis factor-alpha levels among these three groups (P > 0.05). These data suggested magnesium alloy with micro-arc oxidation treatment has no significant effect on RAW264.7 cell viability, and at the same time, reduces the inflammatory reaction.

Key words: biomaterials, tissue-engineered oral materials, magnesium alloy, micro-arc oxidation, macrophages, tumor necrosis factor-alpha, interleukin-6, lipopolysaccharide, cell viability

CLC Number:

R318

Zhuang Yan, Wu Feng, Meng Gai-li, Li Xiao-hui. Magnesium alloy modified by micro-arc oxidation can influence tumor necrosis factor-alpha and interleukin-6 production in macrophages[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.011.

Figures/Tables 1

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