Alkaloids of Buyang Huanwu decoction promote the in vitro proliferation of human umbilical cord blood stem cells

doi:10.3969/j.issn.2095-4344.2013.19.018

Abstract

Abstract:

BACKGROUND: Studies have shown that Buyang Huanwu decoction has protection effect on various cells, but the mechanism is unclear. Some studies have speculated that the alkaloids of Buyang Huanwu decoction play a role.
OBJECTIVE: To observe the effect of alkaloid of Buyang Huanwu decoction on the in vitro proliferation of human umbilical cord blood stem cells.
METHODS: Fresh umbilical cord blood was obtained from healthy puerperant to prepare the human umbilical cord blood stem cells suspension, then the purified human umbilical cord blood stem cells were seeded into 40 culture dishes for passage. The cells were divided into four groups. The cells in the tetramethylpyrazine group were cultured with 100 g/L tetramethylpyrazine, the cells in the ferulic acid group were cultured with 50 g/L ferulic acid and the cells in the alkaloids group (tetramethylpyrazine+ferulic acid group) were cultured with 100 g/L tetramethylpyrazine and 50 g/L ferulic acid; cells in the control group were continuously cultured with Dulbecco’s modified Eagle’s medium containing fetal bovine serum with the volume fraction of 20%. The proliferation of human umbilical cord blood stem cells were observed after cultured for 2, 4, 6, 8 and 10 days; after cultured for 24 and 72 hours, the ratio of human umbilical cord blood stem cells in S phase was determined by flow cytometry, and after cultured for 10 days, the resting membrane potential changes were recorded with current clamp mode.
RESULTS AND CONCLUSION: The proliferation of human umbilical cord blood stem cells in the tetramethylpyrazine group and ferulic acid group was not obvious; after cultured for 10 days, the proliferation of human umbilical cord blood stem cells in the alkaloids group was significantly higher than that in the tetramethylpyrazine group, ferulic acid group and control group (P < 0.01). After cultured for 24 and 72 hours in the alkaloids group, the number of human umbilical cord blood stem cells in S phase was increased; the resting membrane potential in the alkaloids group was higher than that in the tetramethylpyrazine group, ferulic acid group and control group (P < 0.01). The results indicate that the alkaloid of Buyang Huanwu decoction can promote the proliferation of human umbilical cord blood stem cells.

Key words: stem cells, stem cells and traditional Chinese medicine, human umbilical cord blood stem cells, Buyang Huanwu decoction, alkaloid, cell proliferation, tetramethylpyrazine, ferulic acid, resting membrane potential, S phase, flow cytometry, current clamp mode

CLC Number:

R394.2

Cai Yong, Lin Jin-li, Bulinbaiyila. Alkaloids of Buyang Huanwu decoction promote the in vitro proliferation of human umbilical cord blood stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3533-3537.

Figures/Tables 3

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