Synapsin expression of endogenous neural stem cell membrane in a mouse model of amyotrophic lateral sclerosis

doi:10.3969/j.issn.2095-4344.2013.19.017

Abstract

Abstract:

BACKGROUND: Fibroblast growth factor 2 is one of the main neurotrophic factors for nervous system, has been proved to have a role in promoting the differentiation of endogenous neural stem cells.
OBJECTIVE: To detect the improvement of motor behavior and the proliferation of endogenous neural stem cells, the level of synapsin and the correlation between the differentiation number of endogenous neural stem cells and the level of synapsin in the SOD1G93A G1H transgenic mouse model of amyotrophic lateral sclerosis after fibroblast growth factor 2 administration.
METHODS: Sixty new born SOD1G93A G1H transgenic mice (mouse models of amyotrophic lateral sclerosis) were divided into amyotrophic lateral sclerosis group and fibroblast growth factor 2 group, 30 mice in each group. Thirty newborn wild B6SJL mice were collected as normal control group. Mice in the fibroblast growth factor 2 group received intraperitoneal injection of fibroblast growth factor 2; the mice in the amyotrophic lateral sclerosis group and the normal control group received intraperitoneal injection of placebo normal saline. The improvement of motor behavior was evaluated with Rotarod test at postnatal 60, 90 and 120 days; immunohistochemistry was used to mark the endogenous neural stem cells and count the synapsin; the correlation between the differentiation number of endogenous neural stem cells and the level of synapsin was evaluated with Spearman statistical method.
RESULTS AND CONCLUSION: Compared with the amyotrophic lateral sclerosis group, the fibroblast growth factor 2 group showed a statistically significant improvement in the motor behavior, number of endogenous neural stem cells and level of synapsin were significantly increased. There was a negative correlation between the differentiation number of endogenous neural stem cells and level of synapsin. The neuro-protection mechanism of fibroblast growth factor 2 may be associated with the proliferation of endogenous neural stem cells and level of synapsin.

Key words: stem cells, stem cell culture and differentiation, amyotrophic lateral sclerosis, fibroblast growth factor 2, endogenous neural stem cells, neural stem cells, synaptophysin, SOD1^G^93A^G1H transgenic mice, cytokines, motor function, scotch instrument, ministerial grants-supported paper

CLC Number:

R394.2

Ye Xue, Ma Chun-chao, Yao Yang, Zang Da-wei. Synapsin expression of endogenous neural stem cell membrane in a mouse model of amyotrophic lateral sclerosis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3527-3532.

Figures/Tables 4

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