Inter-alpha-trypsin inhibitor level reflects the chronic renal allograft rejection

doi:10.3969/j.issn.2095-4344.2013.18.002

Abstract

Abstract:

BACKGROUND: Currently, the pathological examination of renal transplantation is still the “gold standard” for chronic renal allograft rejection. But the procedural biopsy for renal transplantation is still difficult to spread in China, and there exists a certain risk. Due to various reasons, the patients with chronic rejection miss the timing of treatment as timely detection fails.
OBJECTIVE: To detect the inter-alpha-trypsin inhibitor level in the serum of rats with chronic renal allograft rejection.
METHODS: Normal male Wistar rats were used as donors and normal male Sprague Dawley rats were used as recipients to perform renal transplantation. After transplantation, the rats were divided into chronic rejection group and normal renal transplantation group. Recipents in the chronic rejection group were intraperitoneally injected with cyclosporine A microemulsion 2 mg/kg once a day from 3 days before transplantation, and those in the normal renal transplantation group were intraperitoneally injected with cyclosporine A microemulsion 2 mg/kg daily.
RESULTS AND CONCLUSION: Western blot results showed that the inter-alpha-trypsin inhibitor levels in two groups at 4, 6, 8, 10 and 12 weeks after transplantation were significantly decreased when compared with those at 1 week before transplantation (P < 0.01). At 4 and 6 weeks after transplantation, the inter-alpha-trypsin inhibitor level in the chronic rejection group was higher than that in the normal renal transplantation group (P < 0.01), but lower than that in the normal renal transplantation group at 8, 10 and 12 weeks after transplantation. The inter-alpha-trypsin inhibitor level in the chronic rejection group was decreased in a time-dependent manner (P < 0.01). The results show that the inter-alpha-trypsin inhibitor level is significantly decreased when chronic rejection appears after renal transplantation. The change of inter-alpha-trypsin inhibitor level is closely related with the chronic renal allograft rejection, which can provide basis for the prediction of chronic rejection after renal transplantation.

Key words: organ transplantation, renal transplantation, nter-alpha-trypsin inhibitor, rats, chronic rejection, cyclosporine A, Wistar rat, Sprague Dawley rat, intraperitoneal injection

CLC Number:

R318

Huang Jin-qiu, Zhang Jian-qiang, Hu Jian-min, Chen Chuan-bao, Hu Yu-xiang, Chen Hua, Li Liu-yang, Fan Li-pei, Zhao Ming. Inter-alpha-trypsin inhibitor level reflects the chronic renal allograft rejection[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(18): 3239-3246.

Figures/Tables 2

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