Expression of transforming growth factor beta 1 of cryopreserved skin allograft

doi:10.3969/j.issn.2095-4344.2013.18.001

Abstract

Abstract:

BACKGROUND: Skin transplantation and wound surface coverage are the most important measures for the treatment of wound and burn. At present, allogenic skin still is the best available wound coverage.
OBJECTIVE: To evaluate the application of allogeneic skin in the wound by detecting the expression of transforming growth factor-β1 in rat skin allograft under cryopreservation.
METHODS: The skin specimens of rats were preserved at -20 ℃ (low temperature preservation group) or at
-80℃ (cryopreservation group) for 1 week, 1 month and 2 months, respectively. Then skin pieces in each group were transplanted into the back of rats. Autologous skin grafting rats were as control group.
RESULTS AND CONCLUSION: Compared with low temperature preservation group, the expression of transforming growth factor-β1 in the cryopreservation group was inhibited, rejection of skin allograft was delayed and survival time was prolonged. Histology score of skin rejection was also lower in the cryopreservation group. Compared with low temperature preservation, cryopreservation could reduce expression of antigen of transforming growth factor-β1 in skin allograft. The results show that cryopreserved allograft skin as a kind of wound temporary coverage has wide application prospect in case that skin tissue is inadequate or insufficient.

Key words: organ transplantation, tissue transplantation, skin allograft, cryopreservation, transforming growth factor beta 1, rejection, wound coverage, skin transplantation, provincial grants-supported paper

CLC Number:

R318

Cao Li-ying, Ding Xuan-xi, Yang Sheng-ping, Wang Guan, Jiang Ming-jing. Expression of transforming growth factor beta 1 of cryopreserved skin allograft[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(18): 3231-3238.

Figures/Tables 7

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