Eupolyphaga sinensis-containing serum influences the adipogenic differentiation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.14.019

Abstract

Abstract:

BACKGROUND: The experiments try to explain the pathogenesis of Eupolyphaga sinensis for the treatment of avascular necrosis of femoral head through investigating the effect of Eupolyphaga sinensis on the adipogenic differentiation of hormone-induced bone marrow mesenchymal stem cells.
OBJECTIVE: To investigate the intervention effect of Eupolyphaga sinensis on the adipogenic differentiation of dexamethasone-induced bone marrow mesenchymal stem cells.
METHODS: High-dose dexamethasone was used to induce the adipogenic differentiation of in vitro cultured bone marrow mesenchymal stem cells, and during the adipogenic differentiation, Eupolyphaga sinensis-containing serum was used for intervention. After 6 days intervention, expressions of adipogenic markers, such as triglyceride, peroxisome proliferator-activated receptor mRNA and adipocyte protein 2 mRNA, were detected.
RESULTS AND CONCLUSION: Eupolyphaga sinensis could inhibit the expression of peroxisome proliferator-activated receptor mRNA and adipocyte protein 2 mRNA in the differentiation of bone marrow mesenchymal stem cells, and could reduce the level of triglyceride, which suggested that Eupolyphaga sinensis drug can reverse the increased adipogenic differentiation of bone marrow mesenchymal stem cells caused by high-dose hormones.

Key words: stem cells, stem cells and traditional Chinese medicine, bone marrow mesenchymal stem cells, femoral head necrosis, adipogenic differentiation, traditional Chinese medicine, drug-containing serum, Eupolyphaga sinensis, dexamethasone, hormone, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Qi Zhen-xi, Li Shu-qiang, Yu Tao. Eupolyphaga sinensis-containing serum influences the adipogenic differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2597-2602.

Figures/Tables 6

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