Immune tolerance induced by bone marrow mesenchymal stem cells after islet allotransplantation in diabetic minipigs

doi:10.3969/j.issn.2095-4344.2013.14.013

Abstract

Abstract:

BACKGROUND: Islet transplantation is a promising method used to radically cure diabetes mellitus. The immune rejection caused by transplantation hinders the development of transplantation. The immune tolerance of recipients to donor grafts is a key to solving the problem of allotransplantation rejection reaction.
OBJECTIVE: To investigate the influence of infusion of bone marrow mesenchymal stem cells on the survival time of islet allograft in the minipigs after islet allotransplantation.
METHODS: Under sterile condition, minipig bone marrow was taken and the supernatant was discarded after centrifugation. After washed with L-DMEM culture medium, mononuclear cells were adjusted to be 1×10⁹/L. After 48-hour culture, nonadherent cells were discarded and mesenchymal stem cells were isolated and amplified. Passage 5 cells were taken as donors and cell concentration was adjusted to be 1×10¹⁰/L. Minipig models of alloxan-induced diabetes mellitus were prepared and then randomly divided into two groups: bone marrow cells and bone marrow cells + mesenchymal stem cells. In the bone marrow cells group, islet cells and bone marrow cells were infused. In the bone marrow cells + mesenchymal stem cells group, islet cells, bone marrow cells and bone marrow mesenchymal stem cells were infused.
RESULTS AND CONCLUSION: Compared with infusion of simple bone marrow cells, infusion of bone marrow mesenchymal stem cells and bone marrow cells significantly prolonged the survival time of islet allografts (P < 0.05) and maintained high serum insulin level for a longer time in diabetic minipigs. These findings suggest that bone marrow mesenchymal stem cell infusion can significantly prolong the survival time of islet allografts and contribute to the normal function of islet allografts in diabetic minipigs.

Key words: stem cells, stem cell transplantation, bone marrow mesenchymal stem cells, islet, transplantation, islet allotransplantation, immune tolerance, bone marrow cells, islet cells, minipig model of diabetes mellitus, minipig, National Natural Science Foundation of China, stem cell photographs-containing paper

CLC Number:

R394.2

Yang Rui, Ma Zhao-jie, Chen Rong-ping, Wang Mei, Zhang Zhen, Cai De-hong, Chen Hong. Immune tolerance induced by bone marrow mesenchymal stem cells after islet allotransplantation in diabetic minipigs[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2558-2562.

Figures/Tables 3

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