Recombinant human bone morphogenetic protein-2 adjusts expression of vascular endothelial growth factor in human adipose-derived mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.14.007

Abstract

Abstract:

BACKGROUND: Recombinant bone morphogenetic protein-2 can promote tissue engineering bone vascularization, but its biological rules targeting human cells are not clear. At present, there is in which report on recombinant bone morphogenetic protein-2 adjusts the expression of vascular endothelial growth factor in human cells.
OBJECTIVE: To observe and compare the expression of vascular endothelial growth factor in human adipose-derived mesenchymal stem cells on gene level and protein level at different time points after induced with human recombinant bone morphogenetic protein-2.
METHODS: Adipose-derived mesenchymal stem cells were separated from adult human adipose tissues and cultured until passage 3, then divided into induced group and control group. The cells in the induced group were induced by human recombinant bone morphogenetic protein-2 which final concentration was 100 μg/L, then the samples were collected at 3, 6, 12, 18, 24, 36 and 48 hours after induction. Reverse transcription-PCR and enzyme-linked immunosorbent assay were used to detect vascular endothelial growth factor expression on gene level and protein level, compared with the control group.
RESULTS AND CONCLUSION: Human recombinant bone morphogenetic protein-2 adjusted vascular endothelial growth factor expression of adipose mesenchymal stem cells in a time-dependent manner, and the expression of vascular endothelial growth factor changed at different time points. Compared with the control group, human recombinant bone morphogenetic protein-2 could suppress vascular endothelial growth factor expression at 3-6 hours (P < 0.05), while at 18-24 hours, human recombinant bone morphogenetic protein-2 could promote vascular endothelial growth factor expression (P < 0.05). These two time periods should be paid attention when using human recombinant bone morphogenetic protein-2 to promote tissue engineering bone vascularization.

Key words: stem cells, adipose-derived stem cells, adipose-derived mesenchymal stem cells, human recombinant bone morphogenetic protein-2, vascular endothelial growth factor, time-dependent, gene level, protein level, cell isolation, cell culture, reverse transcription-PCR, enzyme-linked immunosorbent assay, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Cao Xin, Jin Ge-le, Yang Yi, Chen Hui-jin, Yin Jian. Recombinant human bone morphogenetic protein-2 adjusts expression of vascular endothelial growth factor in human adipose-derived mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2517-2524.

Figures/Tables 5

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