Biological characteristics of tumor stem cells from human osteosarcoma cell line MG-63

doi:10.3969/j.issn.2095-4344.2013.10.005

Abstract

Abstract:

BACKGROUND: Osteosarcoma stem cells play a critical role in the occurrence, relapse and drug resistance of osteosarcoma, but there are fewer studies about isolation and culture and biological characteristics of osteosarcoma stem cells in vitro.
OBJECTIVE: To isolate the highly tumorigenic sub-populations of cells with stem cell features from the human osteosarcoma cell line MG-63, and to detect the expression of CD105, a specific surface marker of tumor stem cells．
METHODS: Osteosarcoma cell spheres were isolated from human osteosarcoma cell line MG-63 with serum-free culturing techniques. The stem cell-related markers CD73, CD90 and CD105 in the osteosarcoma cell spheres were detected by using flow cytometry. Immunofluorescence technique was used for detecting the positive rate of CD105 in MG-63 cell line and osteosarcoma cell spheres. CD105⁺and CD105^- cells were separated by magnetic activated cell sorting technique. The tumorigenic potent of CD105⁺ and CD105^-cells were evaluated via the transplantation into NOD/SCID mice.
RESULTS AND CONCLUSION: The high expression of CD105 was observed in osteosarcoma cell spheres, and these cells possessed the property of high invasiveness and high tumorigenesis. Tumor could be induced in NOD-SCID mice by transplantation of CD105 cells at 1 000 cells per mouse．These cells exhibited autonomous growth features and could be cultured in serum-free condition for a long time. It indicates that osteosarcoma cells with the phenotype of CD105 have stem cell features and CD105 may be one of the surface markers of osteosarcoma stem cells.

Key words: stem cells, tumor stem cells, osteosarcoma, cell line, MG-63, CD105, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Feng Da-peng, Huang Quan, Li Yan, Zheng Bi-qiang, Xiao Jian-ru. Biological characteristics of tumor stem cells from human osteosarcoma cell line MG-63[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(10): 1736-1741.

Figures/Tables 6

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