In vitro induction of adipose-derived stem cells into endothelial cells and angiogenic cells

doi:10.3969/j.issn.2095-4344.2013.10.004

Abstract

Abstract:

BACKGROUND: Adipose-derived stem cells, as the seed cells for tissue engineering, can be induced into endothelial cells, thereby solving the key problem of vascularization.
OBJECTIVE: To in vitro investigate the feasibility of inducing adipose-derived stem cells to differentiate into endothelial cells and the blood vessel formation on three-dimensional media.
METHODS: Adipose-derived stem cells were isolated and cultured by enzyme digestion. Passage 3 or 4 adipose-derived stem cells were induced into endothelial cells with conditional medium and then the induced cells were cultured in Matrigel medium. Cell surface antigen CD31 was detected by immunohistochemistry before and after induction. After hematoxylin-eosin staining, cell morphology was observed by phase contrast microscopy.
RESULTS AND CONCLUSIONS: Immunohistochemical examination results showed that CD31 expression was negative in adipose-derived stem cells, but positive expression was detected in the induced cells. The cells cultured in Matrigel media accumulated together gradually, emanated pseudopodia after 24 hours and formed grid structure after 1 week, and long vasculature was observed after 2 weeks. CD31 expression was detected in the induced cells. These results indicate that adipose-derived stem cells can be in vitro induced into endothelial cells, then which form vasculature, suggesting that adipose-derived stem cells can be used as the ideal seed cells for vascularization in tissue-engineered grafts.

Key words: stem cells, adipose-derived stem cells, endothelial cells, vessels, induction, CD31, three-dimensional culture, cell culture, adipose tissue, differentiation, stem cell photographs-containing paper

CLC Number:

R394.2

Ma Li, Huang Lu-gang, Lin Ping. In vitro induction of adipose-derived stem cells into endothelial cells and angiogenic cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(10): 1730-1735.

Figures/Tables 5

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