Clone of human KLF4 gene and construction of recombinant KLF4 lentiviral vector

doi:10.3969/j.issn.2095-4344.2013.07.018

Abstract

Abstract:

BACKGROUND: KLF4 is an essential gene for cell differentiation.
OBJECTIVE: To construct a lentiviral vector pCDH-KLF4 gene.
METHODS: KLF4 gene amplification was used by PCR. Gene amplification products were inserted into the lentiviral vector pCDH and to co-transfect 293TN cells. DNA sequencing was used to confirm the recombinant vector. The virus supernatant was harvested and titrated. The expression of KLF4 was detected by reverse transcription-PCR.
RESULTS AND CONCLUSION: DNA sequencing confirmed that the sequence of amplified KLF4 gene was consistent with the Genebank data. In transfected 5637 cells, KLF4 mRNA was over-expressed. Results verified that lentiviral vevtors of KLF4 gene over-expression were successfully constructed.

Key words: tissue construction, cytology experiments in tissue construction, lentiviral vector, KLF4 gene, gene recombination, 5637 cells, plasmid vector, DNA sequencing, tissue engineering, urinary tract tumors, Escherichia coli, DH5a competent cells, the National Natural Science Foundation of China, tissue construction photographs-containing paper

CLC Number:

R318

Wang Bo-han, Yu Xiao, Yu Gan, Li Heng, Xiao Wei, Xu Hua, Ye Zhang-qun. Clone of human KLF4 gene and construction of recombinant KLF4 lentiviral vector[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1238-1242.

Figures/Tables 4

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