Regulatory effect of decellularized cartilage matrix on macrophage polarization

doi:10.3969/j.issn.2095-4344.3185

Abstract

Abstract: BACKGROUND: Biomaterials derived from extracellular matrix have been successfully applied in the field of tissue engineering regeneration. In recent years, great attention has been paid to the effect of biomaterials on the phenotypic polarization of macrophages.
OBJECTIVE: To investigate the regulatory effects of decellularized cartilage extracellular matrix (DCM) and pepsin-treated decellularized cartilage extracellular matrix (PDCM) on phenotypic polarization of macrophages.
METHODS: The DCM of the pig was prepared by physical comminution, repeated freeze-thaw and differential centrifugation. The biodegradation product (PDCM) was obtained by dissolving it in pepsin solution. Mouse macrophage cell line RAW264.7 was divided into five groups. In the control group, complete macrophage culture medium was added. In the M1 positive control group, lipopolysaccharide + interferon γ solution was added. In the M2 positive control group, interleukin 4 solution was added. In the PDCM group, PDCM solution was added. In the pepsin group, pepsin solution was added. After 18 hours of culture, immunofluorescence staining for CD86 (M1 type macrophages) and CD206 (M2 type macrophages) was performed. In addition, mouse macrophage cell line RAW264.7 was divided into six groups: control group, M1 positive control group, M2 positive control group, pepsin group, DCM coating group and PDCM coating group. After 24 hours of culture, CD86 and CD206 were detected by flow cytometry.
RESULTS AND CONCLUSION: (1) Immunofluorescence results showed that in the PDCM group, induced CD86 expression ratio was approximately 0.13; induced CD206 expression ratio was approximately 0.5. (2) Flow cytometry results showed that DCM induced CD86 (20.1%) and CD206 (28.8%) expression in macrophages; PDCM only induced CD206 expression (23.2%). (3) The results showed that DCM and its biodegradation products could promote M2 polarization of macrophages.

Key words: cartilage, materials, tissue engineering, decellularized, cartilage matrix, macrophage polarization, macrophage phenotype

CLC Number:

Tian Guangzhao, Yang Zhen, Zha Kangkang, Sun Zhiqiang, Li Xu, Sui Xiang, Huang Jingxiang, Guo Quanyi, Liu Shuyun. Regulatory effect of decellularized cartilage matrix on macrophage polarization[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3545-3550.

Figures/Tables 6

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