Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (7): 1243-1250.doi: 10.3969/j.issn.2095-4344.2013.07.019
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He Jing, Wei Si-yu, Liu Feng-yong, Ye Jing, Shen Bing-ling, Yao Xiao-mei
Received:
2012-10-15
Revised:
2012-11-23
Online:
2013-02-12
Published:
2013-02-12
Contact:
Yao Xiao-mei, Ph.D. in neuroscience, Professor, Master’s supervisor, Department of Pathophysiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China
jupx@163.com
About author:
He Jing★, Studying for master’s degree, Department of Pathophysiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China
hjdelightful@163.com
CLC Number:
He Jing, Wei Si-yu, Liu Feng-yong, Ye Jing, Shen Bing-ling, Yao Xiao-mei. Hydrogen peroxide induced oxidative stress in the spleen of metallothionein Ⅰ/Ⅱ knockout mice[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1243-1250.
2.3 NAC能减轻H2O2诱导的金属硫蛋白Ⅰ/Ⅱ敲除鼠脾细胞乳酸脱氢酶活性 乳酸脱氢酶是糖酵解过程中一种重要的酶,任何原因引起的细胞损伤均可导致乳酸脱氢酶漏出,使培养液中乳酸脱氢酶活性增加。 处理2 h后乳酸脱氢酶测定结果显示,与对照组相比,NAC组乳酸脱氢酶活力显著降低(P < 0.01),0.5,1 mmol/L H2O2组培养液上清乳酸脱氢酶活力升高(P < 0.01),但增加10 mmol/L NAC处理后,与0.5 mmol/L H2O2相比,NAC+0.5 mmol/L H2O2组培养液上清乳酸脱氢酶活力明显降低(P < 0.01),与1 mmol/L H2O2相比,NAC+1 mmol/L H2O2组培养液上清乳酸脱氢酶活力也明显降低(P < 0.01),但是NAC+0.5 mmol/L H2O2组和NAC+1 mmol/L H2O2组与对照组相比,乳酸脱氢酶活力仍升高,见表3。"
2.4 NAC能减轻H2O2诱导的金属硫蛋白Ⅰ/Ⅱ敲除鼠脾细胞线粒体通透性转换孔开放和线粒体肿胀 线粒体通透性转换孔开放可对甘露醇和蔗糖高通透造成线粒体膨胀,线粒体吸光度值减少。因而,通过检测线粒体吸光度值的变化可反映线粒体通透性转换孔的开放情况[15]。 处理2 h后测定结果显示,与对照组相比,NAC组线粒体吸光度值升高(P < 0.01),0.5、1 mmol/L H2O2 组线粒体吸光度值降低(P < 0.01),但增加10 mmol/L NAC 处理后,与0.5 mmol/L H2O2相比,NAC+0.5 mmol/L H2O2组线粒体吸光度值显著增加(P < 0.01),与 1 mmol/L H2O2相比,NAC+1 mmol/L H2O2组线粒体吸光度值也显著增加(P < 0.01),NAC+0.5 mmol/L H2O2 组和NAC+1 mmol/L H2O2组与对照组相比,线粒体吸光度值也是增高的,说明NAC能明显改善H2O2(0.5, 1 mmol/L)诱导的线粒体通透性转换孔开放和线粒体肿胀。见表4。"
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