Cardiac function of pigs with myocardial infarction after autologous bone marrow mesenchymal stem cell transplantation

doi:10.3969/j.issn.2095-4344.2013.06.004

Abstract

Abstract:

BACKGROUND: Stem cells transplantation can improve the ischemic myocardial blood supply and improve the cardiac function.
OBJECTIVE: To further identify the application and effect of autologous bone marrow mesenchymal stem cells on the cardiac function after myocardial infarction.
METHODS: Fifteen Taihu Meishan pigs were selected to make the myocardial infarction models, and then divided into four groups (5 swine in each group). At 2 weeks after modeling, the pigs were treated with autologous bone marrow stem cells transplantation. The change in each index of cardiac function was observed with Doppler. The level of serum vascular endothelial growth factor was detected in different periods after transplantation. At the end of the experiment, the general specimens were removed, and the colonization and differentiation of the transplanted cells were detected with immunohistochemistry, and the myocardial vascular density was also detected.
RESULTS AND CONCLUSION: There were no significant differences in ejection fraction, left ventricular internal dimension diastole and left ventricular internal dimension systole, as well as the myocardial vascular density and the level of serum vascular endothelial growth factor at different time points between myocardial infarction 3 hours group and model group. Compared with the model group, the cardiac function indicators were improved in the transplantation group at 2 weeks after transplantation, the myocardial vascular density in the transplantation group was higher than that in the model group, the cardiac function at 2 weeks after myocardial infarction was better than others and the serum vascular endothelial growth factor level was improved after transplantation (P < 0.05). The results suggest that myocardial microenvironment at different time points has different effects on bone marrow mesenchymal stem cell differentiation and colonization, and bone marrow-derived stem cell transplantation in the early scar repairing has positive effects on the improvement of cardiac function and the differentiation and colonization of bone marrow-derived stem cells.

Key words: stem cells, bone marrow-derived stem cells, bone marrow mesenchymal stem cells, myocardial infarction, ischemic myocardial disease, stem cell transplantation, cardiac function, vascular density, vascular endothelial growth factor, differentiation, colonization, pig, stem cell photographs-containing paper

CLC Number:

R394.2

Yu Gui-ping, Shen Zhen-ya, Guo Shi-qiang, Yu Yun-sheng, Chen Guo-qiang. Cardiac function of pigs with myocardial infarction after autologous bone marrow mesenchymal stem cell transplantation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 969-973.

Figures/Tables 5

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